Lee S K, Shaw A, Maher S E, Bothwell A L
Section of Immunobiology, Yale University School of Medicine, New Haven, CT 06520-8011.
Int Immunol. 1994 Oct;6(10):1621-7. doi: 10.1093/intimm/6.10.1621.
To study the role of p59fyn in T cell activation, we used antisense RNA to inhibit p59fyn expression in a T cell clone. Transfectants with reduced levels of p59fyn were functionally impaired in their responses to antigen, Con A+recombinant IL-1 and cross-linking with anti-TCR mAb. Induction of tyrosine phosphorylation on most intracellular substrates was greatly reduced. We also noted that the lck kinase activity was greatly reduced even though the amount of lck protein was equivalent to that present in parental D10 cells. Our results suggest that the protein tyrosine kinase p59fyn is critical in TCR-mediated signaling and also suggests that p59fyn may regulate p56lck tyrosine kinase activity.
为研究p59fyn在T细胞活化中的作用，我们使用反义RNA抑制T细胞克隆中p59fyn的表达。p59fyn水平降低的转染子在对抗原、刀豆蛋白A +重组白细胞介素-1以及与抗TCR单克隆抗体交联的反应中功能受损。大多数细胞内底物上酪氨酸磷酸化的诱导显著减少。我们还注意到，尽管lck蛋白的量与亲本D10细胞中的量相当，但lck激酶活性却大大降低。我们的结果表明，蛋白酪氨酸激酶p59fyn在TCR介导的信号传导中至关重要，也表明p59fyn可能调节p56lck酪氨酸激酶活性。
