Arndt E R, Stevens E S
Department of Chemistry, State University of New York at Binghamton, New York 13902-6000.
Biopolymers. 1994 Nov;34(11):1527-34. doi: 10.1002/bip.360341110.
Agarose films were prepared using a variety of casting conditions, heat treatments, and solvation treatments. Films represented the high temperature sol, the room temperature gel, and a high temperature state with cross-links intact. The vacuum uv CD of the film samples varies considerably, but the similarities between the film CD and the previously reported solution CD show that dehydration per se does not affect the CD. Combining the CD results for the dried sol with the x-ray results of Foord and Atkins leads to a description of the high temperature sol in terms of locally extended chain conformations. The CD of the gel and its peculiarly high uv absorption are satisfactorily, albeit not uniquely, interpreted in terms of the double-helix model of the gel, in agreement with previous conformational studies based on chiroptical properties.
使用多种浇铸条件、热处理和溶剂化处理制备了琼脂糖薄膜。薄膜呈现出高温溶胶、室温凝胶以及交联完整的高温状态。薄膜样品的真空紫外圆二色性有很大差异，但薄膜圆二色性与先前报道的溶液圆二色性之间的相似性表明，脱水本身不会影响圆二色性。将干燥溶胶的圆二色性结果与富德和阿特金斯的X射线结果相结合，得出了关于高温溶胶的局部伸展链构象的描述。凝胶的圆二色性及其特别高的紫外吸收，尽管不是唯一地，但根据凝胶的双螺旋模型得到了令人满意的解释，这与先前基于手性光学性质的构象研究一致。
