噬菌体T4的复制性DNA解旋酶参与DNA重组。
Involvement of a replicative DNA helicase of bacteriophage T4 in DNA recombination.
作者信息
Yonesaki T
机构信息
Department of Biology, College of General Education, Osaka University, Japan.
出版信息
Genetics. 1994 Oct;138(2):247-52. doi: 10.1093/genetics/138.2.247.
Abstract
Bacteriophage T4 gene 41 encodes a replicative DNA helicase that is a subunit of the primosome which is essential for lagging-strand DNA synthesis. A mutation, rrh, was generated and selected in the helicase gene on the basis of limited DNA replication that ceases early. The survival of ultraviolet-irradiated phage and the frequency of genetic recombination are reduced by rrh. In addition, rrh diminishes the production of concatemeric DNA. These results strongly suggest that the gene 41 replicative helicase participates in DNA recombination.
摘要
噬菌体T4基因41编码一种复制性DNA解旋酶，它是引发体的一个亚基，对滞后链DNA合成至关重要。在解旋酶基因中产生并筛选出了一个名为rrh的突变，该突变基于早期停止的有限DNA复制。rrh降低了紫外线照射噬菌体的存活率和基因重组频率。此外，rrh减少了串联DNA的产生。这些结果强烈表明基因41复制性解旋酶参与了DNA重组。
相似文献
1
Involvement of a replicative DNA helicase of bacteriophage T4 in DNA recombination.噬菌体T4的复制性DNA解旋酶参与DNA重组。
Genetics. 1994 Oct;138(2):247-52. doi: 10.1093/genetics/138.2.247.
2
DNA helicase requirements for DNA replication during bacteriophage T4 infection.噬菌体T4感染期间DNA复制对DNA解旋酶的需求。
J Bacteriol. 1994 Mar;176(6):1667-72. doi: 10.1128/jb.176.6.1667-1672.1994.
3
DNA helicase mutants of bacteriophage T4 that are defective in DNA recombination.在DNA重组方面存在缺陷的噬菌体T4的DNA解旋酶突变体。
Mol Gen Genet. 1999 Oct;262(3):525-33. doi: 10.1007/s004380051114.
4
Control of helicase loading in the coupled DNA replication and recombination systems of bacteriophage T4.噬菌体 T4 中耦合的 DNA 复制和重组系统中解旋酶加载的控制。
J Biol Chem. 2014 Jan 31;289(5):3040-54. doi: 10.1074/jbc.M113.505842. Epub 2013 Dec 14.
5
Overexpression, purification, sequence analysis, and characterization of the T4 bacteriophage dda DNA helicase.T4噬菌体dda DNA解旋酶的过表达、纯化、序列分析及特性鉴定
J Biol Chem. 1992 Oct 15;267(29):20674-81.
6
Escherichia coli and Bacillus subtilis PriA proteins essential for recombination-dependent DNA replication: involvement of ATPase/helicase activity of PriA for inducible stable DNA replication.大肠杆菌和枯草芽孢杆菌中参与依赖重组的DNA复制所必需的PriA蛋白：PriA的ATP酶/解旋酶活性对诱导性稳定DNA复制的作用
Biochimie. 1999 Aug-Sep;81(8-9):847-57. doi: 10.1016/s0300-9084(99)00211-4.
7
Plasmid models for bacteriophage T4 DNA replication: requirements for fork proteins.噬菌体T4 DNA复制的质粒模型：对叉蛋白的要求。
J Virol. 1992 Dec;66(12):6960-8. doi: 10.1128/JVI.66.12.6960-6968.1992.
8
Mediator proteins orchestrate enzyme-ssDNA assembly during T4 recombination-dependent DNA replication and repair.介体蛋白在T4重组依赖性DNA复制和修复过程中协调酶与单链DNA的组装。
Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8298-305. doi: 10.1073/pnas.131007498.
9
Conditional coupling of leading-strand and lagging-strand DNA synthesis at bacteriophage T4 replication forks.噬菌体T4复制叉处前导链与后随链DNA合成的条件偶联。
J Biol Chem. 2001 Aug 3;276(31):29559-66. doi: 10.1074/jbc.M101310200. Epub 2001 Jun 4.
10
UvsX recombinase and Dda helicase rescue stalled bacteriophage T4 DNA replication forks in vitro.UvsX重组酶和Dda解旋酶在体外拯救停滞的噬菌体T4 DNA复制叉。
J Biol Chem. 2004 Aug 20;279(34):35735-40. doi: 10.1074/jbc.M403942200. Epub 2004 Jun 11.
引用本文的文献
1
Depth-stratified functional and taxonomic niche specialization in the 'core' and 'flexible' Pacific Ocean Virome.“核心”和“灵活”太平洋病毒群落中的深度分层功能和分类生态位特化
ISME J. 2015 Feb;9(2):472-84. doi: 10.1038/ismej.2014.143. Epub 2014 Aug 5.
2
Bacteriophage T4 genome.噬菌体T4基因组。
Microbiol Mol Biol Rev. 2003 Mar;67(1):86-156, table of contents. doi: 10.1128/MMBR.67.1.86-156.2003.
3
Bacteriophage T4 gene 41 helicase and gene 59 helicase-loading protein: a versatile couple with roles in replication and recombination.噬菌体T4基因41解旋酶和基因59解旋酶加载蛋白：在复制和重组中发挥作用的多功能组合。
Proc Natl Acad Sci U S A. 2001 Jul 17;98(15):8312-8. doi: 10.1073/pnas.121009398.
4
Destabilization of bacteriophage T4 mRNAs by a mutation of gene 61.5.基因61.5突变导致噬菌体T4 mRNA不稳定。
Genetics. 1996 Sep;144(1):7-14. doi: 10.1093/genetics/144.1.7.
本文引用的文献
1
The purification and characterization of gene 59 protein from bacteriophage T4.来自噬菌体T4的基因59蛋白的纯化与特性分析
J Biol Chem. 1994 Jan 14;269(2):1284-9.
2
DNA helicase requirements for DNA replication during bacteriophage T4 infection.噬菌体T4感染期间DNA复制对DNA解旋酶的需求。
J Bacteriol. 1994 Mar;176(6):1667-72. doi: 10.1128/jb.176.6.1667-1672.1994.
3
Participation of bacteriophage T4 gene 41 in replication repair.噬菌体T4基因41在复制修复中的作用。
Mutat Res. 1980 Apr;70(2):131-8. doi: 10.1016/0027-5107(80)90152-9.
4
Arrangement of the single-stranded fragments in E. coli bacteriophage BF23 DNA.大肠杆菌噬菌体BF23 DNA中单链片段的排列
Gene. 1980 Mar;8(4):345-68. doi: 10.1016/0378-1119(80)90041-4.
5
Studies on the recombination genes of bacteriophage T4: suppression of uvsX and uvsY mutations by uvsW mutations.噬菌体T4重组基因的研究：uvsW突变对uvsX和uvsY突变的抑制作用
Genetics. 1987 Feb;115(2):219-27. doi: 10.1093/genetics/115.2.219.
6
The essential role of recombination in phage T4 growth.
Annu Rev Genet. 1987;21:347-71. doi: 10.1146/annurev.ge.21.120187.002023.
7
DNA sequencing with Thermus aquaticus DNA polymerase and direct sequencing of polymerase chain reaction-amplified DNA.使用嗜热栖热菌DNA聚合酶进行DNA测序以及对聚合酶链反应扩增的DNA进行直接测序。
Proc Natl Acad Sci U S A. 1988 Dec;85(24):9436-40. doi: 10.1073/pnas.85.24.9436.
8
T4 phage gene uvsX product catalyzes homologous DNA pairing.T4噬菌体基因uvsX产物催化同源DNA配对。
EMBO J. 1985 Dec 1;4(12):3321-7. doi: 10.1002/j.1460-2075.1985.tb04083.x.
9
Synergistic action of three recombination gene products of bacteriophage T4, uvsX, uvsY, and gene 32 proteins.
J Biol Chem. 1989 May 15;264(14):7814-20.
Zotero 插件