McConnell S J, Kendall M L, Reilly T M, Hoess R H
DuPont Merck Pharmaceutical Company, Experimental Station, Wilmington, DE 19980-0328.
Gene. 1994 Dec 30;151(1-2):115-8. doi: 10.1016/0378-1119(94)90640-8.
The monoclonal antibody (mAb) KAA8 recognizes the peptide angiotensin II (AII). The KAA8 mAb was biopanned using two phage-displayed peptide libraries, one unconstrained, the other constrained by a disulfide bond. After several cycles of biopanning, both libraries showed enrichment for phage that bind KAA8. Phage isolated from the unconstrained library contain a consensus sequence that matches the sequence of AII. A consensus sequence was also identified from the constrained library that does not resemble the AII sequence, and represents a mimotope of AII. We have also demonstrated that monovalent phage display can be used to discriminate between modest and high-affinity binding peptides.
单克隆抗体（mAb）KAA8可识别肽血管紧张素II（AII）。使用两个噬菌体展示肽库对KAA8单克隆抗体进行生物淘选，一个无限制，另一个由二硫键限制。经过几个生物淘选循环后，两个文库均显示出与KAA8结合的噬菌体富集。从无限制文库中分离出的噬菌体包含与AII序列匹配的共有序列。还从受限制文库中鉴定出一个与AII序列不同的共有序列，它代表AII的模拟表位。我们还证明了单价噬菌体展示可用于区分中等亲和力和高亲和力结合肽。
