嗜热栖热放线菌DNA连接酶编码基因序列及DNA连接酶中的保守基序。
Sequence of the DNA ligase-encoding gene from Thermus scotoductus and conserved motifs in DNA ligases.
作者信息
Jónsson Z O, Thorbjarnardóttir S H, Eggertsson G, Palsdottir A
机构信息
Laboratory of Molecular Genetics, University of Iceland, Reykjavik.
出版信息
Gene. 1994 Dec 30;151(1-2):177-80. doi: 10.1016/0378-1119(94)90652-1.
By dideoxynucleotide sequencing of a genomic clone, we have determined the complete nucleotide sequence of the gene encoding NAD(+)-dependent DNA ligase (EC 6.5.1.2) of the thermophilic bacterium Thermus scotoductus. The gene encodes a 674-amino-acid thermostable enzyme highly similar to other bacterial DNA ligases and to parts of the deduced gene product of Escherichia coli ORF f562, 5' to the spoR gene encoding 5' guanosyl kinase.
通过对一个基因组克隆进行双脱氧核苷酸测序，我们确定了嗜热细菌嗜热栖热放线菌（Thermus scotoductus）中编码NAD⁺依赖性DNA连接酶（EC 6.5.1.2）的基因的完整核苷酸序列。该基因编码一种674个氨基酸的耐热酶，它与其他细菌DNA连接酶以及大肠杆菌ORF f562（位于编码5'-鸟苷激酶的spoR基因5'端）推导的基因产物的部分区域高度相似。
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