Olive M, Thézé N, Philippe M, Le Pennec J P, Lerivray H
Université de Rennes I, Unité CNRS No. 256, France.
Gene. 1994 Dec 30;151(1-2):81-8. doi: 10.1016/0378-1119(94)90635-1.
CDK2 (cyclin-dependent kinase 2) is a serine/threonine kinase which is involved in regulating S-phase entry in higher eukaryotes. To investigate the transcriptional control of this gene, a 13-kb Xenopus laevis genomic clone containing the 5' flanking sequences was isolated. A 2.7-kb fragment containing the promoter region was sequenced and the transcription start point (tsp) was determined by primer extension. Several putative regulatory elements, such as the E2F-binding site, Y box and octamer-binding site, were localized in this region, but no TATA box was found. When fused to cat, a reporter gene encoding chloramphenicol acetyltransferase, the 5' flanking sequences were shown to function in oocytes and an enhancer activity was found in this region. During early embryogenesis, cdk2 promoter activity was tested and de novo transcription was detected at the mid-blastula transition.
细胞周期蛋白依赖性激酶2（CDK2）是一种丝氨酸/苏氨酸激酶，参与调控高等真核生物进入S期。为了研究该基因的转录调控，分离出了一个包含5'侧翼序列的13 kb非洲爪蟾基因组克隆。对包含启动子区域的2.7 kb片段进行测序，并通过引物延伸确定转录起始点（tsp）。该区域定位了几个假定的调控元件，如E2F结合位点、Y盒和八聚体结合位点，但未发现TATA盒。当与编码氯霉素乙酰转移酶的报告基因cat融合时，5'侧翼序列在卵母细胞中显示出功能，并且在该区域发现了增强子活性。在早期胚胎发育过程中，检测了cdk2启动子活性，并在囊胚中期转变时检测到了从头转录。
