Chan P, Simon-Chazottes D, Mattei M G, Guenet J L, Salier J P
Institute National de la Santé et de la Recherche Médicale, Unit-78, Boisguillaume, France.
Genomics. 1994 Sep 1;23(1):145-50. doi: 10.1006/geno.1994.1470.
The lipocalin superfamily encompasses a large set of quite distantly related proteins that act as carriers for small, lipophilic molecules. The lipocalin genes coding for orosomucoid, the alpha 1-microglobulin/bikunin precursor, and the major urinary protein map to MMU4, while their human counterparts map to the homologous HSA9q34 area where three other lipocalin genes for complement C8 gamma chain (C8G), progestagen-associated endometrial protein (PAEP), and prostaglandin D synthase (PTGDS) are also located. By linkage analyses in an interspecific backcross progeny in mouse, the Lcn2 gene coding for the oncogenic lipocalin 24p3, as well as the 3 lipocalin genes for C8G, PTGDS, and PAEP, have now been assigned to MMU2. The first three genes map to proximal MMU2, which is known to be homologous to HSA9q34. Paep is more distally located, which extends the number of regions with conserved syntenies between HSA9q34 and MMU2. By in situ hybridization, the human LCN2 gene maps to HSA9q34. Our data indicate that the lipocalin locus arrangement in the human/mouse ancestor is closer to that found at HSA9q than to that in the MMU genome.
脂质运载蛋白超家族包含一大组亲缘关系较远的蛋白质，它们作为亲脂性小分子的载体发挥作用。编码血清类黏蛋白、α1 - 微球蛋白/ bikunin前体和主要尿蛋白的脂质运载蛋白基因定位于MMU4，而它们在人类中的对应基因定位于同源的HSA9q34区域，补体C8γ链（C8G）、孕激素相关子宫内膜蛋白（PAEP）和前列腺素D合成酶（PTGDS）的另外三个脂质运载蛋白基因也位于该区域。通过对小鼠种间回交后代的连锁分析，编码致癌脂质运载蛋白24p3的Lcn2基因以及C8G、PTGDS和PAEP的3个脂质运载蛋白基因现已定位于MMU2。前三个基因定位于MMU2近端，已知该区域与HSA9q34同源。Paep的位置更靠远端，这扩展了HSA9q34和MMU2之间具有保守同线性的区域数量。通过原位杂交，人类LCN2基因定位于HSA9q34。我们的数据表明，人类/小鼠祖先中的脂质运载蛋白基因座排列更接近HSA9q上的排列，而不是MMU基因组中的排列。
