Yamamoto T, Horiguchi H, Kamma H, Noro M, Ogata T, Inage Y, Akaogi E, Mitsui K, Hori M, Isobe M
Department of Pathology, University of Tsukuba.
Jpn J Cancer Res. 1994 Nov;85(11):1171-7. doi: 10.1111/j.1349-7006.1994.tb02924.x.
To determine whether image cytometry (ICM) is advantageous for clinical DNA analyses of tumor cells, nuclear DNA contents measured by ICM were compared with those by flow cytometry (FCM), using 46 samples of non-small cell lung cancers. ICM was performed on smear specimens of fresh materials (f-ICM) and cell suspensions obtained from paraffin-embedded tumors (p-ICM). The same cell suspensions were also analyzed by FCM (p-FCM). Aneuploid rates/coefficient of variation (CV) of f-ICM, p-ICM, and p-FCM were 76.1/4.90, 71.7/5.01 and 60.9/5.31%, respectively. There was a high correlation in the DNA indices between p-ICM and p-FCM (r = 0.80). In the comparative DNA analysis, there were seven discordant samples. Six of them were estimated as aneuploid by p-ICM, but they were miscounted as diploid or undefinable (impossible) by p-FCM. This was caused by measuring condensed nuclei or debris. All "impossible" samples in p-FCM were squamous cell carcinoma with necrosis. In cell cycle analysis, the S and S+G2/M phase fractions in diploid samples were higher in p-ICM than those in p-FCM (P < 0.005), because the G0/G1 phase (2N) fraction presented by FCM was composed of cancer and non-malignant cells in diploid cancers. In ICM, they can be separately measured by means of morphological selection. These findings indicated that ICM is superior to FCM, especially for the practical DNA measurement of a few cancer cells and in the evaluation of the proliferation rates.
为了确定图像细胞术（ICM）在肿瘤细胞临床DNA分析中是否具有优势，我们使用46例非小细胞肺癌样本，将ICM测量的核DNA含量与流式细胞术（FCM）测量的结果进行了比较。ICM是在新鲜材料的涂片标本（f-ICM）和从石蜡包埋肿瘤中获得的细胞悬液（p-ICM）上进行的。相同的细胞悬液也通过FCM进行分析（p-FCM）。f-ICM、p-ICM和p-FCM的非整倍体率/变异系数（CV）分别为76.1/4.90、71.7/5.01和60.9/5.31%。p-ICM和p-FCM之间的DNA指数具有高度相关性（r = 0.80）。在比较DNA分析中，有7个不一致的样本。其中6个样本通过p-ICM估计为非整倍体，但通过p-FCM误计为二倍体或无法确定（不可能）。这是由于测量浓缩核或碎片所致。p-FCM中所有“不可能”的样本均为伴有坏死的鳞状细胞癌。在细胞周期分析中，二倍体样本中p-ICM的S期和S+G2/M期分数高于p-FCM（P < 0.005），因为FCM呈现出的G0/G1期（2N）分数由二倍体癌症中的癌细胞和非恶性细胞组成。在ICM中，可以通过形态学选择分别测量它们。这些发现表明，ICM优于FCM，特别是在对少数癌细胞进行实际DNA测量和评估增殖率方面。
