从人类蜱叮咬部位培养伯氏疏螺旋体：感染风险指南。
Cultivation of Borrelia burgdorferi from human tick bite sites: a guide to the risk of infection.
作者信息
Berger B W, Johnson R C, Kodner C, Coleman L
机构信息
Department of Dermatology, State University of New York at Stony Brook.
出版信息
J Am Acad Dermatol. 1995 Feb;32(2 Pt 1):184-7. doi: 10.1016/0190-9622(95)90123-x.
BACKGROUND
The risk of acquiring Lyme disease has been evaluated by xenodiagnostic procedures with laboratory strains of Borrelia burgdorferi and laboratory-reared Ixodes ticks, or by clinical trials in which diagnosis was based on clinical findings, culture, or serologic tests.
OBJECTIVE
Our purpose was to determine the risk of infection from tick bites in a natural setting in which wild strains of B. burgdorferi were involved, by a biopsy culture technique.
METHODS
Skin biopsy specimens were obtained from Ixodes scapularis tick bite sites, processed, and examined for the presence of B. burgdorferi.
RESULTS
B. burgdorferi was cultivated from only 2 of 48 skin biopsy specimens. In both instances duration of tick attachment was approximately 24 hours.
CONCLUSION
In a hyperendemic region for Lyme disease the risk of infection after a deer tick bite appears to be low, particularly if the tick has been attached for less than 24 hours.
背景
通过使用伯氏疏螺旋体实验室菌株和实验室饲养的蜱进行接种诊断程序，或通过基于临床发现、培养或血清学检测进行诊断的临床试验，对感染莱姆病的风险进行了评估。
目的
我们的目的是通过活检培养技术，确定在涉及伯氏疏螺旋体野生菌株的自然环境中蜱叮咬导致感染的风险。
方法
从肩胛硬蜱叮咬部位获取皮肤活检标本，进行处理，并检查是否存在伯氏疏螺旋体。
结果
48份皮肤活检标本中仅2份培养出伯氏疏螺旋体。在这两个病例中，蜱附着的持续时间约为24小时。
结论
在莱姆病高度流行地区，鹿蜱叮咬后感染的风险似乎较低，尤其是如果蜱附着时间少于24小时。
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