Malley A, Zeleny-Pooley M, Murray G
Oregon Regional Primate Research Center, Beaverton 97006.
J Immunol Methods. 1995 Jan 13;178(1):31-9. doi: 10.1016/0022-1759(94)00237-q.
H-2 specific T suppressor inducer (Tsfi) and T suppressor effector (Tsfe) factors show a dose-dependent inhibition of one-way mixed lymphocyte responses (MLR) between CBA/J responder spleen cells and C57BL/6 mitomycin C-treated stimulator spleen cells. The hydrophobic proteins Tsfi and Tsfe purified by ammonium sulfate precipitation and affinity methods were stabilized by the addition of Tris-saline pH 8 buffered octylglucopyranoside solution. The stabilized Tsfi and Tsfe fractions stored at 4 degrees C for 3-7 months retained a significant (> 72%) amount of their ability to inhibit MLR. Tsfi and Tsfe purified by salt precipitation and affinity methods were analyzed by SDS-PAGE. Enzyme-linked immunoassay (ELISA) and Western blots indicated that these molecules had T cell receptor (TcR) alpha chain, I-J, and IL-10 epitopes, but not TcR beta chain epitopes.
H-2特异性T抑制诱导因子（Tsfi）和T抑制效应因子（Tsfe）对CBA/J应答脾细胞与经丝裂霉素C处理的C57BL/6刺激脾细胞之间的单向混合淋巴细胞反应（MLR）呈现剂量依赖性抑制作用。通过硫酸铵沉淀和亲和方法纯化的疏水蛋白Tsfi和Tsfe，通过添加pH 8的Tris-盐水缓冲的辛基吡喃葡萄糖苷溶液得以稳定。在4℃下储存3至7个月的稳定化Tsfi和Tsfe组分保留了显著（>72%）的抑制MLR的能力。通过盐沉淀和亲和方法纯化的Tsfi和Tsfe经SDS-PAGE分析。酶联免疫吸附测定（ELISA）和蛋白质免疫印迹表明，这些分子具有T细胞受体（TcR）α链、I-J和IL-10表位，但不具有TcRβ链表位。
