Vile J M, D'Souza U M, Strange P G
Research School of Biosciences, University of Kent, Canterbury, England, UK.
J Neurochem. 1995 Feb;64(2):940-3. doi: 10.1046/j.1471-4159.1995.64020940.x.
[3H]Nemonapride and [3H]spiperone are very widely used to study dopaminergic systems in vitro and in vivo, but it has been reported that [3H]nemonapride and [3H]spiperone give markedly different Bmax values for preparations of D2 dopamine receptors from recombinant cell lines or animal tissues. We have used the two radioligands in parallel to study a range of dopamine receptors [D2(short), D2(long), and D3] in different buffers. Bmax values derived using either radioligand differ by an average of < 20%, independent of receptor type or buffer conditions. All competition experiments show that the two ligands compete at a single site. It seems that [3H]spiperone and [3H]nemonapride do not differentiate between different forms or populations of D2-like receptors.
[3H]奈莫必利和[3H]螺哌隆被广泛用于体外和体内研究多巴胺能系统，但有报道称，对于重组细胞系或动物组织制备的D2多巴胺受体，[3H]奈莫必利和[3H]螺哌隆给出的Bmax值明显不同。我们同时使用这两种放射性配体，在不同缓冲液中研究一系列多巴胺受体[D2（短）、D2（长）和D3]。使用任一放射性配体得出的Bmax值平均相差<20%，与受体类型或缓冲液条件无关。所有竞争实验表明，这两种配体在单一位点竞争。看来[3H]螺哌隆和[3H]奈莫必利无法区分不同形式或群体的D2样受体。
