Cass W A, Gerhardt G A
Department of Anatomy and Neurobiology, MN 224 Chandler Medical Center, University of Kentucky, Lexington 40536.
Neurosci Lett. 1994 Aug 1;176(2):259-63. doi: 10.1016/0304-3940(94)90096-5.
In vivo electrochemistry was used to determine the effects of locally applied raclopride (a D2 receptor antagonist) and SCH-23390 (a D1 receptor antagonist) on the clearance of locally applied dopamine in the striatum, nucleus accumbens, and medial prefrontal cortex of rats. Chronoamperometric recordings were continuously made at 5 Hz using Nafion-coated, single carbon fiber electrodes. When a calibrated amount of dopamine was pressure ejected at 5-min intervals from a micropipette adjacent (280-310 microns) to the electrode, transient and reproducible dopamine signals were detected in all three regions. Local application of raclopride from a second micropipette, prior to pressure ejection of dopamine, increased the amplitude and time course of the dopamine signals, indicating significant inhibition of the dopamine transporter. In contrast, local application of SCH-23390 or saline had no effect on the dopamine signals. These data indicate that D2, but not D1, dopamine receptors can modulate the activity of the dopamine transporter.
采用体内电化学方法，以确定局部应用雷氯必利（一种D2受体拮抗剂）和SCH-23390（一种D1受体拮抗剂）对大鼠纹状体、伏隔核和内侧前额叶皮质中局部应用多巴胺清除的影响。使用涂有Nafion的单碳纤维电极，以5 Hz的频率连续进行计时电流记录。当每隔5分钟从与电极相邻（280 - 310微米）的微量移液器中压力喷射校准量的多巴胺时，在所有三个区域均检测到瞬态且可重复的多巴胺信号。在压力喷射多巴胺之前，从第二个微量移液器局部应用雷氯必利，增加了多巴胺信号的幅度和时程，表明多巴胺转运体受到显著抑制。相比之下，局部应用SCH-23390或生理盐水对多巴胺信号无影响。这些数据表明，D2多巴胺受体而非D1多巴胺受体可调节多巴胺转运体的活性。
