Jonas R E, Yuan C, Ebrey T G
Center for Biophysics and Computational Biology, University of Illinois, Urbana 61801.
Prep Biochem. 1994 Nov;24(3-4):279-88. doi: 10.1080/10826069408010099.
The transducin heterotrimer and its alpha- and beta gamma-subunits have been purified from frozen bovine rod outer segments by modifying existing procedures. The methods described here are relatively simple and fast. The yield (ca. 8 mgs/100 retinas) and purity of the transducin heterotrimer and subunits from frozen retinas is equal to or larger than those previously obtained from fresh or frozen retinas.
通过改进现有方法，已从冷冻牛视杆细胞外段中纯化出转导素异源三聚体及其α和βγ亚基。本文所述方法相对简单且快速。从冷冻视网膜中获得的转导素异源三聚体及其亚基的产量（约8毫克/100个视网膜）和纯度与之前从新鲜或冷冻视网膜中获得的产量和纯度相当或更高。
