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干扰素γ和主要组织相容性复合体编码亚基对人多催化蛋白酶肽酶活性的影响。

Effects of interferon gamma and major histocompatibility complex-encoded subunits on peptidase activities of human multicatalytic proteases.

作者信息

Ustrell V, Pratt G, Rechsteiner M

机构信息

Department of Biochemistry, University of Utah School of Medicine, Salt Lake City 84132.

出版信息

Proc Natl Acad Sci U S A. 1995 Jan 17;92(2):584-8. doi: 10.1073/pnas.92.2.584.

DOI:10.1073/pnas.92.2.584
PMID:7831334
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC42786/
Abstract

We have examined several peptidase activities of human multicatalytic protease (MCP) purified from the lymphoblastoid cell line 721.45 and a deletion mutant derivative, 721.174, lacking MCP subunits encoded in the major histocompatibility complex (MHC) class II region. Wild-type lymphoblast MCP hydrolyzed a specific peptide, glutaryl-Gly-Gly-Phe-4-methylcoumaryl-7-amide (-MCA), several times faster than the mutant enzyme did, suggesting that MHC-encoded subunits may provide this activity. Contrary to a recent report [Driscoll, J., Brown, M. G., Finley, D. & Monaco, J J. (1993) Nature (London) 365, 262-264], we did not detect significant aminopeptidase associated with lymphoblast MCPs. Our results also differ markedly from those of Gaczynska et al. [Gaczynska, M., Rock, K. L. & Goldberg, A L. (1993) Nature (London) 365, 264-267], who reported that gamma interferon (IFN-gamma) alters the peptidase activities of lymphoblast MCPs. We found that IFN-gamma did not produce significant differences in the peptidase activities of purified MCPs. Moreover, our measurements of Vmax and Km for succinyl-Leu-Leu-Val-Tyr-MCA hydrolysis differ 600-fold and 15-fold, respectively, from those reported by Gaczynska et al. On balance, the findings presented here do not support the idea that IFN-gamma induces major changes in the peptidase activity of purified MCPs.

摘要

我们检测了从淋巴母细胞系721.45中纯化得到的人多催化蛋白酶（MCP）以及一个缺失突变体衍生物721.174的几种肽酶活性。721.174缺失主要组织相容性复合体（MHC）II类区域编码的MCP亚基。野生型淋巴母细胞MCP水解特定肽段戊二酰 - 甘氨酰 - 苯丙氨酸 - 4 - 甲基香豆素 - 7 - 酰胺（-MCA）的速度比突变酶快几倍，这表明MHC编码的亚基可能提供这种活性。与最近的一份报告[德里斯科尔，J.，布朗，M.G.，芬利，D.和莫纳科，J.J.（1993年）《自然》（伦敦）365，262 - 264]相反，我们未检测到与淋巴母细胞MCP相关的显著氨肽酶活性。我们的结果也与加钦斯卡等人[加钦斯卡，M.，罗克，K.L.和戈德堡，A.L.（1993年）《自然》（伦敦）365，264 - 267]的结果明显不同，他们报告γ干扰素（IFN - γ）会改变淋巴母细胞MCP的肽酶活性。我们发现IFN - γ对纯化的MCP的肽酶活性没有产生显著差异。此外，我们对琥珀酰 - 亮氨酰 - 亮氨酰 - 缬氨酰 - 酪氨酸 - MCA水解的Vmax和Km测量值与加钦斯卡等人报告的值分别相差600倍和15倍。总体而言，此处呈现的研究结果不支持IFN - γ会诱导纯化的MCP的肽酶活性发生重大变化这一观点。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d12/42786/ac90d41e1a12/pnas01480-0254-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d12/42786/eb158a18c94b/pnas01480-0252-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d12/42786/ac90d41e1a12/pnas01480-0254-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d12/42786/eb158a18c94b/pnas01480-0252-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/9d12/42786/ac90d41e1a12/pnas01480-0254-a.jpg

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本文引用的文献

1
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2
Peptides naturally presented by MHC class I molecules.由MHC I类分子天然呈递的肽段。
Annu Rev Immunol. 1993;11:213-44. doi: 10.1146/annurev.iy.11.040193.001241.
3
The multicatalytic and 26 S proteases.多催化蛋白酶和26S蛋白酶。
Age (Dordr). 2014 Feb;36(1):57-72. doi: 10.1007/s11357-013-9543-x. Epub 2013 May 22.
4
The Ubiquitin-Proteasome System in Huntington's Disease: Are Proteasomes Impaired, Initiators of Disease, or Coming to the Rescue?亨廷顿舞蹈病中的泛素-蛋白酶体系统：蛋白酶体是受损了、是疾病的引发者，还是前来救援的？
Biochem Res Int. 2012;2012:837015. doi: 10.1155/2012/837015. Epub 2012 Sep 24.
5
The role of the proteasome in the generation of MHC class I ligands and immune responses.蛋白酶体在 MHC I 类配体产生和免疫反应中的作用。
Cell Mol Life Sci. 2011 May;68(9):1491-502. doi: 10.1007/s00018-011-0657-y. Epub 2011 Mar 9.
6
The ubiquitin-like protein FAT10 mediates NF-kappaB activation.泛素样蛋白 FAT10 介导 NF-κB 的激活。
J Am Soc Nephrol. 2010 Feb;21(2):316-26. doi: 10.1681/ASN.2009050479. Epub 2009 Dec 3.
7
Efficient generation of a hepatitis B virus cytotoxic T lymphocyte epitope requires the structural features of immunoproteasomes.高效生成乙型肝炎病毒细胞毒性T淋巴细胞表位需要免疫蛋白酶体的结构特征。
J Exp Med. 2000 Feb 7;191(3):503-14. doi: 10.1084/jem.191.3.503.
8
The proteasome activator 11 S REG (PA28) and class I antigen presentation.蛋白酶体激活剂11S REG（PA28）与I类抗原呈递。
Biochem J. 2000 Jan 1;345 Pt 1(Pt 1):1-15.
9
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10
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4
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8
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9
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10
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J Biol Chem. 1994 Aug 12;269(32):20727-32.