Ramsay A J, Leong K H, Boyle D, Ruby J, Ramshaw I A
Viral Engineering and Cytokine Research Group, John Curtin School of Medical Research, Australian National University, Canberra.
Reprod Fertil Dev. 1994;6(3):389-92. doi: 10.1071/rd9940389.
The expression of the genes for murine interleukin-5 (IL-5) or IL-6 in recombinant vaccinia virus vectors markedly increased IgA reactivity to co-expressed heterologous antigen in the lungs of mice inoculated intranasally with the viruses. These elevated local IgA responses reached a peak four times higher than those elicited by control viruses 14 days after infection and these peak levels were maintained for at least four weeks. Elevated IgA responses, reaching a peak 3-4 weeks after immunization, were also observed in the lungs of mice inoculated with IL-6 expressed by another vector, fowlpox virus. The results indicate that these factors enhance the development of mucosal IgA reactivity in vivo and suggest that their expression in mucosal vaccine vectors may stimulate local immune responses. The approach described in this study may be useful in stimulating mucosal immunity to a wide range of vector-encoded antigens, not only for vaccination against disease but also for immunocontraception by the co-expression of antigens involved in reproduction.
在重组痘苗病毒载体中，小鼠白细胞介素-5（IL-5）或IL-6基因的表达显著增强了经鼻接种这些病毒的小鼠肺部对共表达的异源抗原的IgA反应性。这些升高的局部IgA反应在感染后14天达到峰值，比对照病毒引发的反应高四倍，并且这些峰值水平至少维持四周。在用另一种载体禽痘病毒表达的IL-6接种的小鼠肺部，也观察到免疫后3-4周达到峰值的IgA反应升高。结果表明，这些因子在体内增强了粘膜IgA反应性的发展，并表明它们在粘膜疫苗载体中的表达可能刺激局部免疫反应。本研究中描述的方法可能有助于刺激对多种载体编码抗原的粘膜免疫，不仅用于疾病疫苗接种，还可通过共表达生殖相关抗原用于免疫避孕。
