选择性培养基和培养环境对A群链球菌分离的影响。
Effects of selective media and atmosphere of incubation on the isolation of group A streptococci.
作者信息
Murray P R, Wold A D, Schreck C A, Washington JA I I
出版信息
J Clin Microbiol. 1976 Jul;4(1):54-6. doi: 10.1128/jcm.4.1.54-56.1976.
Abstract
The effects of selective media and atmosphere of incubation on the isolation of group A beta-hemolytic streptococci were evaluated. Sheep blood agar medium with gentamicin (2.5 or 5.0 mug/ml) was inferior to antibiotic-free sheep blood agar medium. This resulted from the partial restriction of group A streptococcal growth on gentamicin medium. Recovery of beta-hemolytic streptococci from specimens in air, CO2, or anaerobic incubation was evaluated. The isolation of group A streptococci was equivalent in the three incubation atmospheres; however, non-group A beta- hemolytic streptococci were isolated significantly more often from specimens incubated in an anaerobic or CO2 atmosphere than from those incubated in air. Therefore, sheep blood agar medium, stabbed with a wire loop and incubated in air, is recommended for the isolation of group A streptococci from throat swabs.
摘要
评估了选择性培养基和培养环境对A组β溶血性链球菌分离的影响。含庆大霉素（2.5或5.0μg/ml）的羊血琼脂培养基不如不含抗生素的羊血琼脂培养基。这是由于庆大霉素培养基对A组链球菌生长的部分限制所致。评估了在空气、二氧化碳或厌氧培养条件下从标本中回收β溶血性链球菌的情况。在三种培养环境中，A组链球菌的分离效果相当；然而，与在空气中培养的标本相比，从厌氧或二氧化碳环境中培养的标本中分离出非A组β溶血性链球菌的频率明显更高。因此，建议使用用金属丝环穿刺并在空气中培养的羊血琼脂培养基从咽拭子中分离A组链球菌。
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