Miller L G, Fahey J M
Department of Pharmacology and Experimental Therapeutics, Tufts University School of Medicine, Boston, Massachusetts 02111.
Ann N Y Acad Sci. 1994 Oct 31;739:292-8. doi: 10.1111/j.1749-6632.1994.tb19831.x.
Interleukin-1 is a polypeptide cytokine involved in the acute-phase response. We examined the effects of IL-1 on the major inhibitory and excitatory neurotransmitter systems in brain mediated by GABA and glutamate, respectively. IL-1 enhanced the effects of GABA as determined by GABA-dependent chloride uptake at 1 and 10 ng/ml in cortical synaptoneurosomes. This effect was attenuated by pretreatment with the IL-1 receptor antagonist. Neither IL-6 nor TNF was effective in this system. IL-1 decreased the effect of NMDA and glycine on intracellular calcium concentrations in cultured chick cortical neurons in both the presence and absence of the endogenous polyamine spermine. This effect was attenuated by pretreatment with the AMPA/kainate antagonist CNQX. These data indicate that IL-1 modulates both inhibitory and excitatory neurotransmitter function in brain.
白细胞介素-1是一种参与急性期反应的多肽细胞因子。我们分别研究了白细胞介素-1对大脑中由γ-氨基丁酸（GABA）和谷氨酸介导的主要抑制性和兴奋性神经递质系统的影响。在皮质突触体中，通过GABA依赖性氯离子摄取测定，1纳克/毫升和10纳克/毫升的白细胞介素-1增强了GABA的作用。用白细胞介素-1受体拮抗剂预处理可减弱这种作用。白细胞介素-6和肿瘤坏死因子（TNF）在该系统中均无效。在有无内源性多胺精胺的情况下，白细胞介素-1均降低了N-甲基-D-天冬氨酸（NMDA）和甘氨酸对培养的鸡皮质神经元细胞内钙浓度的影响。用α-氨基-3-羟基-5-甲基-4-异恶唑丙酸/海人藻酸（AMPA/kainate）拮抗剂CNQX预处理可减弱这种作用。这些数据表明，白细胞介素-1调节大脑中的抑制性和兴奋性神经递质功能。
