大肠杆菌二氢二吡啶甲酸合酶：通过电喷雾质谱法对亚胺中间体和溴丙酮酸处理产物的表征
Escherichia coli dihydrodipicolinate synthase: characterization of the imine intermediate and the product of bromopyruvate treatment by electrospray mass spectrometry.
作者信息
Borthwick E B, Connell S J, Tudor D W, Robins D J, Shneier A, Abell C, Coggins J R
机构信息
Department of Biochemistry, University of Glasgow, U.K.
出版信息
Biochem J. 1995 Jan 15;305 ( Pt 2)(Pt 2):521-4. doi: 10.1042/bj3050521.
Abstract
Simplified procedures for assaying and purifying dihydrodipicolinate synthase (EC 4.2.1.52), the first enzyme of the lysine biosynthetic pathway, have been developed and electrospray ionization m.s. has been used to observe the imine intermediate with pyruvate and to investigate the reaction of the enzyme with bromopyruvate and fluoropyruvate.
摘要
已开发出用于分析和纯化二氢二吡啶甲酸合酶（EC 4.2.1.52，赖氨酸生物合成途径的首个酶）的简化程序，并使用电喷雾电离质谱法来观察与丙酮酸形成的亚胺中间体，以及研究该酶与溴丙酮酸和氟丙酮酸的反应。
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