Odaka A, Tsukahara T, Momoi M, Momoi T
Division of Development and Differentiation, National Institute of Neuroscience, Tokyo, Japan.
Biochem Biophys Res Commun. 1995 Jan 26;206(3):821-8. doi: 10.1006/bbrc.1995.1117.
Three alternative splicing products of amyloid precursor protein (APP), APP770, 751 and 695, were detected in mouse embryonal carcinoma (EC) P19 cells by reverse transcriptase RNA polymerase chain reaction (RT-PCR). Alternative splicing of APP pre-mRNA in P19EC cells was remarkably changed by c-jun transformation. The relative ratio of APP770 encoding exons 7 and 8, non-neuron type, was increased by c-jun transformation, while that of APP 695 not encoding exons 7 and 8, neuron-specific one, was decreased. These results suggested that skipping of exons 7 and 8 was specifically blocked in c-jun transformed cells. APP 695, which increases in P19 EC cells under the culture conditions that induce the neuronal differentiation, did not increase in C2C5 cells under the same conditions, suggesting that c-jun transformed cells were not in the neuronal cell lineage and lost the ability to differentiate into neurons.
通过逆转录酶RNA聚合酶链反应（RT-PCR）在小鼠胚胎癌细胞（EC）P19细胞中检测到淀粉样前体蛋白（APP）的三种可变剪接产物，即APP770、751和695。c-jun转化显著改变了P19EC细胞中APP前体mRNA的可变剪接。c-jun转化增加了编码第7和第8外显子的APP770（非神经元型）的相对比例，而未编码第7和第8外显子的APP 695（神经元特异性）的相对比例则降低。这些结果表明，在c-jun转化的细胞中，第7和第8外显子的跳跃被特异性阻断。在诱导神经元分化的培养条件下，P19 EC细胞中增加的APP 695在相同条件下的C2C5细胞中并未增加，这表明c-jun转化的细胞不在神经元细胞谱系中，并且失去了分化为神经元的能力。
