Nonradioactive northwestern analysis using biotinylated riboprobes.

A nonradioactive modification of the Northwestern assay is described and applied to the detection of RNA-binding proteins. The nonradioactive assay is based on the use of biotinylated riboprobes, which are stable and easy to handle. Chemiluminescence is generated with streptavidin-conjugated peroxidase and provides even better sensitivity than the radioactive detection method to assay RNA binding to proteins bound to nitrocellulose.