Rodríguez P L, Carrasco L
Universidad Autonoma de Madrid, Spain.
Biotechniques. 1994 Oct;17(4):702, 704, 706-7.
A nonradioactive modification of the Northwestern assay is described and applied to the detection of RNA-binding proteins. The nonradioactive assay is based on the use of biotinylated riboprobes, which are stable and easy to handle. Chemiluminescence is generated with streptavidin-conjugated peroxidase and provides even better sensitivity than the radioactive detection method to assay RNA binding to proteins bound to nitrocellulose.
