Lee L H, Baglin T
Department of Haematology, Addenbrooke's NHS Trust, Cambridge.
Br J Haematol. 1995 Jan;89(1):131-6. doi: 10.1111/j.1365-2141.1995.tb08899.x.
Platelet phospholipid dependent thrombin generation was determined in 20 patients with thrombocytosis, 18 with thrombocytopenia, and 25 normal controls, using a quantitative chromogenic assay. Platelets from patients with myeloproliferative disorders displayed normal lag times to 20 nM thrombin concentration but increased thrombin potentials, even when corrected for platelet size. Platelets from patients with reactive thrombocytosis supported normal thrombin generation. ITP platelets were large, with a proportionate increase in thrombin potentials, but very short lag times to 20 nM thrombin concentration. Following marrow ablation there was a progressive loss of activation-induced enhancement of thrombin generation. Platelets from patients with idiopathic aplastic anaemia supported normal thrombin generation. These findings indicate that platelet phospholipid-dependent thrombin generation is altered in many patients with a variety of quantitative platelet disorders, and this may be an important determinant of the clinical expression of these disorders.
采用定量显色分析法，对20例血小板增多症患者、18例血小板减少症患者及25名正常对照者进行了血小板磷脂依赖性凝血酶生成检测。骨髓增殖性疾病患者的血小板对20 nM凝血酶浓度的滞后时间正常，但凝血酶生成潜力增加，即便校正了血小板大小也是如此。反应性血小板增多症患者的血小板支持正常的凝血酶生成。免疫性血小板减少症（ITP）患者的血小板体积较大，凝血酶生成潜力相应增加，但对20 nM凝血酶浓度的滞后时间极短。骨髓消融后，激活诱导的凝血酶生成增强作用逐渐丧失。特发性再生障碍性贫血患者的血小板支持正常的凝血酶生成。这些发现表明，许多患有各种血小板数量异常疾病的患者，其血小板磷脂依赖性凝血酶生成发生了改变，这可能是这些疾病临床表现的一个重要决定因素。
