Gomeza J, Zafra F, Olivares L, Giménez C, Aragón C
Centro de Biología Molecular Severo Ochoa, Facultad de Ciencias, Universidad Autónoma de Madrid, C.S.I.C., Spain.
Biochim Biophys Acta. 1995 Jan 26;1233(1):41-6. doi: 10.1016/0005-2736(94)00249-o.
The high-affinity glycine transporter in neurons and glial cells is the primary means of inactivating synaptic glycine. The effects of 12-O-tetradecanoylphorbol ester (TPA), a potent activator of protein kinase C (PKC), on the high-affinity Na(+)-dependent glycine transport were investigated in C6 cells, a cell line of glial origin. Incubation of C6 cells with TPA led to concentration- and time-dependent decrease in the glycine transport that could be completely suppressed by the addition of the PKC inhibitor staurosporine. The TPA effect could be mimicked by oleoylacetylglycerol and exogenous phospholipase C. Northern and Western blot analysis indicate that C6 cells express the GLYT1 glycine transporter. Incubation of COS cells transiently transfected with a full-length clone of the GLYT1 transporter in the presence of TPA, produces a decrease in glycine uptake.
神经元和神经胶质细胞中的高亲和力甘氨酸转运体是使突触甘氨酸失活的主要方式。在源自神经胶质的C6细胞中，研究了蛋白激酶C（PKC）的强效激活剂12 - O - 十四烷酰佛波醇酯（TPA）对高亲和力钠依赖性甘氨酸转运的影响。用TPA孵育C6细胞导致甘氨酸转运呈浓度和时间依赖性降低，加入PKC抑制剂星形孢菌素可完全抑制这种降低。油酰乙酰甘油和外源性磷脂酶C可模拟TPA的作用。Northern和Western印迹分析表明C6细胞表达GLYT1甘氨酸转运体。在TPA存在的情况下，用GLYT1转运体的全长克隆瞬时转染COS细胞，会导致甘氨酸摄取减少。
