恶性疟原虫疟色素的分析

Analysis of malaria pigment from Plasmodium falciparum.

作者信息

Hempelmann E, Marques H M

机构信息

Department of Experimental and Clinical Pharmacology, University of the Witwatersrand, Parktown, Johannesburg, South Africa.

出版信息

J Pharmacol Toxicol Methods. 1994 Sep;32(1):25-30. doi: 10.1016/1056-8719(94)90013-2.

DOI:10.1016/1056-8719(94)90013-2

PMID:7833503

Abstract

The biochemical pathway for the production of malaria pigment (haemozoin) forms a fundamental difference between host and parasite and is likely to be an important drug target. A simple method for the isolation of malaria pigment is described. The resultant product retained the in vivo crystalline appearance of pigment as judged by polarizing microscopy. Conditions were found for the disaggregation and separation of pigment. As malaria pigment can adsorb drugs, haem, and iron, such separation techniques are useful tools for studies on the impairment of haemoglobin digestion and pigment formation by antimalarials.

摘要

疟色素（疟原虫血色素）产生的生化途径构成了宿主与寄生虫之间的根本差异，并且很可能是一个重要的药物靶点。本文描述了一种分离疟色素的简单方法。通过偏光显微镜判断，所得产物保留了色素在体内的晶体外观。还发现了色素解聚和分离的条件。由于疟色素可以吸附药物、血红素和铁，此类分离技术是研究抗疟药对血红蛋白消化和色素形成的损害的有用工具。