Guo Y J, Cui D J, Tian Y
Department of medicine, 304th hospital.
Zhonghua Jie He He Hu Xi Za Zhi. 1994 Aug;17(4):217-9, 254.
In this study we determined the glucocorticoid receptor (GR) level (using 3H-dexamethasone as a ligand) and phospholipase A2 (PLA2) activity of lung tissue in guinea pig asthma models at 1st, 3rd, 7th and 14th day of asthma attacks. The results showed that the GR maximal binding capacity (Bmax, fmol/mg pro.) were 54.36 +/- 26.57, 30.99 +/- 10.01, 19.40 +/- 10.06, and 29.16 +/- 11.35; GR dissociation constant (Kd, nmol/L) were 12.73 +/- 4.06, 8.28 +/- 3.23, 5.70 +/- 2.91, and 6.40 +/- 2.64 respectively; and PLA2 actisis (U) were 181.58 +/- 41.50, 239.80 +/- 61.15, 243.08 +/- 31.35, and 235.71 +/- 86.13 respectively. The GR Bmax, Kd and PLA2 of actisis control group were 61.48 +/- 27.60 (fmol/mg pro.), 13.11 +/- 2.88 (nmol/L), 81.06 +/- 15.97 (U). The GR Bmax and Kd of asthmatic animals decreased significantly since the 3rd day, but the PLA2 activity increased significantly since the 1st day as compared to the control group (P < 0.01). The changes of GR Bmax had significant negative correlation with those of the PLA2 activity (r = -0.9023, P < 0.05). The mechanism of the GR and PLA2 actisis changes of asthma animal models were discussed.
在本研究中，我们测定了豚鼠哮喘模型在哮喘发作第1、3、7和14天肺组织中的糖皮质激素受体（GR）水平（以3H-地塞米松作为配体）和磷脂酶A2（PLA2）活性。结果显示，GR最大结合容量（Bmax，fmol/mg蛋白）分别为54.36±26.57、30.99±10.01、19.40±10.06和29.16±11.35；GR解离常数（Kd，nmol/L）分别为12.73±4.06、8.28±3.23、5.70±2.91和6.40±2.64；PLA2活性（U）分别为181.58±41.50、239.80±61.15、243.08±31.35和235.71±86.13。对照组的GR Bmax、Kd和PLA2活性分别为61.48±27.60（fmol/mg蛋白）、13.11±2.88（nmol/L）、81.06±15.97（U）。与对照组相比，哮喘动物自第3天起GR Bmax和Kd显著降低，但自第1天起PLA2活性显著升高（P<0.01）。GR Bmax的变化与PLA2活性的变化呈显著负相关（r=-0.9023，P<0.05）。本文讨论了哮喘动物模型GR和PLA2活性变化的机制。
