Gut A, Shiel N, Kay P M, Segal I, Braganza J M
Gastrointestinal Unit, Baragwanath Hospital, Johannesburg, South Africa.
Clin Chim Acta. 1994 Oct 31;230(2):189-99. doi: 10.1016/0009-8981(94)90271-2.
Four indices of free radical activity were measured in fasting serum/plasma samples from 14 consecutive blacks with clinically quiescent chronic pancreatitis and 15 outwardly healthy hospital personnel at Soweto, the township near Johannesburg in South Africa. The patients had higher serum levels than did controls of lipid isomerisation (P < 0.002) and peroxidation (P < 0.05) markers, with lower plasma levels of glutathione (P < 0.0001) and bioactive fraction of vitamin C (P < 0.002). Lipid peroxide and non-bioavailable vitamin C concentrations in Sowetan patients were significantly higher than in their counterparts from Manchester, UK (P < 0.0001, P < 0.0005, respectively). These differences mirrored those in controls in that outwardly healthy Sowetans had much higher serum lipid peroxide levels than Manchester controls (P < 0.001) and much lower plasma concentration of vitamin C (P < 0.001) and hence of the bioavailable fraction ascorbate (P < 0.0002). Heightened free radical activity is thus a common denominator in chronic pancreatitis irrespective of geography, or putative aetiological factors whether alcoholism or idiopathic, since that ratio was approximately 95:5 at Johannesburg and 50:50 at Manchester. The further finding of subclinical oxidative stress in Sowetan controls and the endemic nature of chronic pancreatitis in that area supports the hypothesis that oxidative stress may be involved in its pathogenesis.
对来自南非约翰内斯堡附近索韦托的14例临床静止期慢性胰腺炎黑人患者和15名外表健康的医院工作人员的空腹血清/血浆样本，测量了四项自由基活性指标。与对照组相比，患者的脂质异构化（P < 0.002）和过氧化（P < 0.05）标志物的血清水平更高，而血浆谷胱甘肽水平（P < 0.0001）和维生素C的生物活性部分水平（P < 0.002）更低。索韦托患者的脂质过氧化物和非生物可利用维生素C浓度显著高于英国曼彻斯特的对照组（分别为P < 0.0001和P < 0.0005）。这些差异在对照组中也有体现，即外表健康的索韦托人的血清脂质过氧化物水平远高于曼彻斯特的对照组（P < 0.001），而血浆维生素C浓度（P < 0.001）以及因此生物可利用的抗坏血酸部分浓度（P < 0.0002）则低得多。因此，无论地理位置如何，也无论假定的病因是酒精中毒还是特发性，自由基活性增强都是慢性胰腺炎的一个共同特征，因为在约翰内斯堡该比例约为95:5，在曼彻斯特为50:50。在索韦托对照组中进一步发现亚临床氧化应激以及该地区慢性胰腺炎的地方病性质，支持了氧化应激可能参与其发病机制的假说。
