Malm T, Bowald S, Bylock A, Busch C, Saldeen T
Department of Thoracic and Cardiovascular Surgery, University Hospital, Uppsala, Sweden.
Eur Surg Res. 1994;26(5):298-308. doi: 10.1159/000129349.
Absorbable, nonwoven patches made from polyhydroxybutyrate (PHB) were implanted as transannular patches into the right ventricular outflow tract and pulmonary artery in 13 weanling sheep, the test group. Six sheep, in which a transannular Dacron patch was implanted, served as control group. The test and the control patches were harvested 3-24 months later. The results were documented macroscopically, by histological examination, and by scanning electron microscopy. The 6-keto-PGF1 alpha activity was measured in the regenerated tissue and compared with that in the native pulmonary artery. No aneurysms were noted. Regeneration of a neointima and a neomedia, comparable to native arterial tissue, was observed in the test group. In the control group, a neointimal layer was present but no neomedia comparable to native arterial tissue. Scanning electron microscopy disclosed differences in the endothelial structure between the test and control groups. 6-keto-PGF1 alpha activity was present in both the test and control groups. It is concluded that absorbable, nonwoven patches of PHB can be used as a scaffold for tissue regeneration in low-pressure systems. The regenerated vessel had structural and biochemical qualities in common with the native pulmonary artery.
将由聚羟基丁酸酯（PHB）制成的可吸收非织造补片作为经环补片植入13只断奶绵羊（试验组）的右心室流出道和肺动脉。6只植入经环涤纶补片的绵羊作为对照组。3至24个月后取出试验组和对照组的补片。通过宏观检查、组织学检查和扫描电子显微镜记录结果。测定再生组织中的6-酮-前列腺素F1α活性，并与天然肺动脉中的活性进行比较。未发现动脉瘤。试验组观察到新生内膜和新生中膜再生，与天然动脉组织相当。对照组存在新生内膜层，但没有与天然动脉组织相当的新生中膜。扫描电子显微镜揭示了试验组和对照组在内皮结构上的差异。试验组和对照组均存在6-酮-前列腺素F1α活性。得出结论，PHB可吸收非织造补片可作为低压系统中组织再生的支架。再生血管具有与天然肺动脉相同的结构和生化特性。
