Benbernou N, Nauciel C
Laboratoire de Microbiologie, Faculté de Médecine de Paris-Ouest, Université Paris, Garches, France.
Immunology. 1994 Oct;83(2):245-9.
Interferon-gamma (IFN-gamma) is known to play a major role in resistance to Salmonella typhimurium infection. In this study, the IFN-gamma production in spleens of mice infected with S. typhimurium was analysed at the single cell level using an ELISPOT method. The in vivo IFN-gamma production during the early phase of infection with virulent and avirulent S. typhimurium strains was examined in four mouse strains. Data show that infection with a virulent strain of S. typhimurium caused a much greater enhancement in the frequency of IFN-gamma-producing cells in innately resistant (ltyr) mice (CBA and DBA/2) than in susceptible (ltys) mice (C57BL/6 and BALB/c). In contrast, infection with an avirulent strain of S. typhimurium induced a clear increase in the number of IFN-gamma-producing cells in susceptible mice which was even greater than in resistant ones. These results indicate that both the host genetic background and bacterial virulence play a critical role in the regulation of IFN-gamma production during the early phase of S. typhimurium infection.
已知γ干扰素（IFN-γ）在抵抗鼠伤寒沙门氏菌感染中起主要作用。在本研究中，使用ELISPOT方法在单细胞水平分析了感染鼠伤寒沙门氏菌的小鼠脾脏中IFN-γ的产生情况。在四种小鼠品系中检测了感染强毒株和无毒株鼠伤寒沙门氏菌早期阶段的体内IFN-γ产生情况。数据显示，与易感（ltys）小鼠（C57BL/6和BALB/c）相比，感染强毒株鼠伤寒沙门氏菌导致先天抗性（ltyr）小鼠（CBA和DBA/2）中产生IFN-γ的细胞频率有更大幅度的提高。相反，感染无毒株鼠伤寒沙门氏菌会使易感小鼠中产生IFN-γ的细胞数量明显增加，甚至比抗性小鼠中的增加幅度更大。这些结果表明，宿主遗传背景和细菌毒力在鼠伤寒沙门氏菌感染早期阶段IFN-γ产生的调节中都起着关键作用。
