阿片类药物可调节系膜细胞的迁移、铺展和黏附。

Opioids modulate migration, spreading and adherence of mesangial cells.

作者信息

Singhal P C, Abramovici M, Bansal M, Jaffer S, Mattana J, Shah R, Gibbons N

机构信息

Department of Medicine, Long Island Jewish Medical Center, New Hyde Park, N.Y. 11042.

出版信息

Nephron. 1994;68(3):366-71. doi: 10.1159/000188401.

DOI:10.1159/000188401

PMID:7838261

Abstract

Glomerular mesangial cells are considered to be modified smooth muscle cells and seem to play a role in the maintenance of glomerular hemodynamics. The present study was carried out to determine the effect of opioids on adhesiveness, spreading and migration of mesangial cells. Morphine enhanced spreading of mesangial cells at early time periods (5 mini, control 8 +/- 2% vs. morphine 15 +/- 1%, p < 0.05; 15 min, control 21 +/- 5% vs. morphine 38 +/- 2%, p < 0.05) as well as at later time periods when compared to control cells (at 2 h, control 23 +/- 1% vs. morphine 49 +/- 1%, p < 0.001; at 3 h, control 28 +/- 3% vs. morphine 63 +/- 2%, p < 0.05). beta-Endorphin also enhanced (p < 0.001) spreading of mesangial cells (at 2 h, control 23 +/- 1% vs. beta-endorphin 48 +/- 3%; at 3 h, control 28 +/- 3% vs. beta-endorphin 65 +/- 1%). Morphine decreased adhesion of mesangial cells to the plastic substrate at 24 h as well at 48 h when compared to the control cells. Naloxone attenuated the effect of morphine on adhesion to the substrate. Morphine enhanced (p < 0.05) migration (percentage of denuded area covered by mesangial cells when compared to control cells (control 26.07 +/- 1.08% vs. morphine 37.5 +/- 2.94%; n = 9). Since the morphine-induced decreased adhesiveness could be attenuated by naloxone, this effect of morphine on mesangial cells appears to be mediated by opioid receptors.

摘要

肾小球系膜细胞被认为是一种特殊的平滑肌细胞，似乎在维持肾小球血流动力学中发挥作用。本研究旨在确定阿片类药物对系膜细胞黏附、铺展和迁移的影响。吗啡在早期（5分钟时，对照组8±2%，吗啡组15±1%，p<0.05；15分钟时，对照组21±5%，吗啡组38±2%，p<0.05）以及与对照细胞相比的后期（2小时时，对照组23±1%，吗啡组49±1%，p<0.001；3小时时，对照组28±3%，吗啡组63±2%，p<0.05）均增强了系膜细胞的铺展。β-内啡肽也增强了（p<0.001）系膜细胞的铺展（2小时时，对照组23±1%，β-内啡肽组48±3%；3小时时，对照组28±3%，β-内啡肽组65±1%）。与对照细胞相比，吗啡在24小时和48小时时均降低了系膜细胞对塑料底物的黏附。纳洛酮减弱了吗啡对底物黏附的影响。吗啡增强了（p<0.05）迁移（与对照细胞相比，系膜细胞覆盖的剥脱面积百分比，对照组26.07±1.08%，吗啡组37.5±2.94%；n = 9）。由于纳洛酮可减弱吗啡诱导的黏附性降低，吗啡对系膜细胞的这种作用似乎是由阿片受体介导的。