Tamura S, Shoji Y, Hasiguchi K, Aizawa C, Kurata T
Department of Pathology, National Institute of Health, Tokyo, Japan.
Vaccine. 1994 Oct;12(13):1238-40. doi: 10.1016/0264-410x(94)90250-x.
Effects of cholera toxin B subunits supplemented with 0.1% cholera toxin (CTB*) on systemic IgE antibody responses to ovalbumin (OVA) were examined in BDFI (H-2b/d), Balb/c (H-2d) and C3H (H-2k) mice given OVA intragastrically or intranasally. Two successive doses of OVA intragastrically administered to Balb/c and C3H mice induced little IgE response and resulted in almost complete unresponsiveness to subsequent intraperitoneal challenge with OVA in Al(OH)3, while the intragastric administration to BDF1 mice induced high IgE response and resulted in abrogation of the unresponsiveness to the subsequent challenge. The intranasal administration of OVA induced little IgE response and suppressed response to the subsequent challenge in any strain of mice. On the other hand, two successive doses of intragastric or intranasal OVA together with CTB* enhanced IgE response in all three strains and the subsequent challenge with OVA in Al(OH)3 induced higher IgE responses. These results suggest that CTB* augments IgE response to OVA and abrogates the unresponsiveness when administered orally or intranasally into mice together with OVA, regardless of the H-2 haplotype of the mice.
在经口或经鼻给予卵清蛋白（OVA）的BDFI（H-2b/d）、Balb/c（H-2d）和C3H（H-2k）小鼠中，研究了补充0.1%霍乱毒素的霍乱毒素B亚基（CTB*）对OVA全身性IgE抗体反应的影响。向Balb/c和C3H小鼠经口连续给予两剂OVA诱导出很少的IgE反应，并导致对随后用氢氧化铝佐剂的OVA腹腔攻击几乎完全无反应，而向BDF1小鼠经口给药诱导出高IgE反应，并消除了对随后攻击的无反应性。经鼻给予OVA在任何品系小鼠中诱导出很少的IgE反应，并抑制了对随后攻击的反应。另一方面，经口或经鼻连续给予两剂OVA与CTB一起增强了所有三个品系中的IgE反应，并且随后用氢氧化铝佐剂的OVA攻击诱导出更高的IgE反应。这些结果表明，当与OVA一起经口或经鼻给予小鼠时，CTB增强了对OVA的IgE反应并消除了无反应性，而与小鼠的H-2单倍型无关。
