Hertzberg H, Guscetti F, Bödeker D, Pospischil A, Eckert J
Institute of Parasitology, University of Zürich, Switzerland.
Zentralbl Veterinarmed B. 1994 Jun;41(4):237-48. doi: 10.1111/j.1439-0450.1994.tb00224.x.
A technique is described for the examination of the abomasum of adult sheep via a rumen fistula. The size of the fistula allowed a manual exploration of the rumen which was necessary to achieve an access to the abomasum. In four adult sheep, two of them infected with Haemonchus contortus, fibreoptic endoscopy was used to investigate the abomasal mucosa macroscopically as well as histopathologically and ultrastructurally by means of mucosal biopsy specimen. The acidity of the abomasal contents was measured directly by introducing an electrode into the abomasum or indirectly in abomasal fluid which was obtained via a stomach catheter through the rumen fistula. The measured values in all four sheep ranged between pH2 and 4. The fibreoptic examination of the abomasum did not influence the plasma pepsinogen and gastrin levels. The possibility of combining histological, serological and coprological data in follow-up studies on the pathophysiology of trichostrongylid infections in the abomasum of individual sheep seems to be useful for the investigation of primary and secondary infections and may contribute to a reduction of the number of experimental animals in such studies.
本文描述了一种通过瘤胃瘘管检查成年绵羊皱胃的技术。瘘管的大小允许对瘤胃进行手动探查，这是进入皱胃所必需的。在四只成年绵羊中，其中两只感染了捻转血矛线虫，通过纤维内窥镜对皱胃黏膜进行宏观检查，并通过黏膜活检标本进行组织病理学和超微结构检查。通过将电极插入皱胃直接测量皱胃内容物的酸度，或通过胃导管经瘤胃瘘管获取皱胃液间接测量。所有四只绵羊的测量值在pH2至4之间。对皱胃的纤维内窥镜检查未影响血浆胃蛋白酶原和胃泌素水平。在个体绵羊皱胃中捻转血矛线虫感染病理生理学的后续研究中，结合组织学、血清学和粪便学数据的可能性，似乎有助于原发性和继发性感染的研究，并可能有助于减少此类研究中的实验动物数量。
