西班牙犬冠状病毒（CCV）的特征。流行病学方面。

Canine-coronavirus (CCV) characterization in Spain. Epidemiological aspects.

作者信息

Martin-Calvo M, Marcotegui M A, Simarro I

机构信息

Dpto. Patalogia Animal I, Facultad de Veterinaria, Universidad Compultense, Madrid, Spain.

出版信息

Zentralbl Veterinarmed B. 1994 Jun;41(4):249-56. doi: 10.1111/j.1439-0450.1994.tb00225.x.

DOI:10.1111/j.1439-0450.1994.tb00225.x

PMID:7839745

Abstract

In this paper the characterization of a canine-coronavirus (CCV) strain isolated in Spain is reported. The CCV cellular-infection cycle on A-72 cells was studied using electron-microscopy techniques. The isolate was found to have similar electrophoretic profile and antigenic properties to an American reference strain. Sera samples were obtained from several canine populations and tested for viral antibodies using two immunoenzymatic methods. Sera specificity was confirmed using immunoblot analysis. The agreement between both ELISA tests produced adequate results (kappa value = 0.64).

摘要

本文报道了在西班牙分离出的一种犬冠状病毒（CCV）毒株的特性。利用电子显微镜技术研究了CCV在A - 72细胞上的细胞感染周期。发现该分离株与一株美国参考毒株具有相似的电泳图谱和抗原特性。从多个犬类群体采集血清样本，使用两种免疫酶法检测病毒抗体。通过免疫印迹分析确认血清特异性。两种酶联免疫吸附测定（ELISA）试验之间的一致性产生了足够的结果（kappa值 = 0.64）。