Kodjo A, Exbrayat P, Richard Y
Ecole Nationale Vétérinaire de Lyon, Marcy l'Etoile, France.
Zentralbl Veterinarmed B. 1994 Jul;41(5):336-43. doi: 10.1111/j.1439-0450.1994.tb00236.x.
Eight Moraxella bovis strains isolated from bovine pink eye, 16 Moraxella bovis related strains isolated from healthy goats nasal flora, one nonhemolytic Moraxella sp. isolated from bovine conjunctivitis and different collection strains of Moraxella and Branhamella genus were studied through the combined use of qualitative genetic transformation assay and the ability to grow on a moraxella bovis defined medium (medium MB). Crude DNA extracted from the strains studied were used to transform two mutant auxotroph competent strains of Moraxella bovis CIP 103741 and CIP 103743. Non-specific positive transformation was obtained with mutant assay strain CIP 103743 when treated with DNA from caprine or bovine Moraxella strains, collection strains of Moraxella bovis, Moraxella lacunata, Moraxella nonliquefaciens and strains of Branhamella genus, whereas specific transformation was observed with mutant assay CIP 103741 when treated only with DNA from all the Moraxella bovis (collection and clinical isolates) and 14 of the 16 caprine Moraxella bovis related strains. The specificity and the simplicity of the test make it suitable for use in clinical laboratories.
对从牛红眼病中分离出的8株牛莫拉菌、从健康山羊鼻腔菌群中分离出的16株牛莫拉菌相关菌株、从牛结膜炎中分离出的1株非溶血性莫拉菌属细菌以及莫拉菌属和布兰汉菌属的不同收集菌株，通过定性遗传转化试验和在牛莫拉菌限定培养基（培养基MB）上的生长能力进行联合研究。从所研究菌株中提取的粗DNA用于转化两株牛莫拉菌CIP 103741和CIP 103743的突变营养缺陷型感受态菌株。当用来自山羊或牛莫拉菌菌株、牛莫拉菌收集菌株、腔隙莫拉菌、非液化莫拉菌和布兰汉菌属菌株的DNA处理时，突变检测菌株CIP 103743获得了非特异性阳性转化，而当仅用来自所有牛莫拉菌（收集菌株和临床分离株）以及16株山羊牛莫拉菌相关菌株中的14株的DNA处理时，突变检测CIP 103741观察到特异性转化。该试验的特异性和简易性使其适用于临床实验室。
