Daini O A, Olukoya D K, Akinrimisi E O, Odugbemi T
Genetics Division, National Institute for Medical Research, Yaba, Lagos.
Afr J Med Med Sci. 1993 Jun;22(2):39-41.
As part of an epidemiological study, R plasmids coding for tetracycline resistance were isolated from enteropathogenic Escherichia coli. A 1.4kb Pst 1 fragment of one of them p1479 (size 5.7kb) has been cloned into plasmid pGL101. This recombinant plasmid containing the tetracycline gene was then transformed into E. coli DHI where the tetracycline resistant gene was fully expressed. Attempts to develop this clone to a diagnostic probe is now in progress.
作为一项流行病学研究的一部分，编码四环素抗性的R质粒从致病性大肠杆菌中分离出来。其中一个质粒p1479（大小为5.7kb）的1.4kb Pst 1片段已被克隆到质粒pGL101中。然后将这个含有四环素基因的重组质粒转化到大肠杆菌DHI中，四环素抗性基因在其中得以充分表达。目前正在尝试将这个克隆开发成一种诊断探针。
