Schwachtgen J L, Ferreira V, Meyer D, Kerbiriou-Nabias D
Hôpital de Bicêtre, Le Kremlin-Bicêtre, France.
Biotechniques. 1994 Nov;17(5):882-7.
A method for efficient transfection of human umbilical vein endothelial cells (HUVEC) is presented here. The procedure involves cell synchronization followed by electroporation and allows the detection of the activity of low-strength promoters. Sodium butyrate, added to the culture medium after electroporation, strongly potentiates the transcriptional activity of viral promoters. The method can be successfully applied to analyze the transcriptional activity of different promoters linked to reporter genes or to express foreign genes controlled by viral promoters.
本文介绍了一种高效转染人脐静脉内皮细胞（HUVEC）的方法。该程序包括细胞同步化，然后进行电穿孔，并能检测低强度启动子的活性。电穿孔后添加到培养基中的丁酸钠可强烈增强病毒启动子的转录活性。该方法可成功应用于分析与报告基因相连的不同启动子的转录活性，或表达由病毒启动子控制的外源基因。
