Bianco P R, Weinstock G M
University of Texas Medical School, Houston.
Biotechniques. 1994 Nov;17(5):974-80.
We describe a modification of an automated kinetic assay for beta-galactosidase (beta-gal) activity. This modification includes an assay to quantitate the amount of protein added to each assay. The determination of specific activity includes the amount of protein in the calculation which produces a specific activity with units of pmol product produced/minute/mg protein. In addition to this modification, we present a series of macros written in Microsoft Excel for either the Macintosh or Windows on the PC. These macros decrease the amount of time required to analyze the data from beta-gal assays.
我们描述了一种用于β-半乳糖苷酶（β-gal）活性的自动动力学测定法的改进方法。这种改进包括一种用于定量每次测定中添加蛋白质数量的测定法。比活性的测定在计算中纳入了蛋白质的量，从而得出以每分钟每毫克蛋白质产生的皮摩尔产物为单位的比活性。除了这种改进之外，我们还展示了一系列用微软Excel为苹果机或个人电脑的Windows系统编写的宏。这些宏减少了分析β-gal测定数据所需的时间。
