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咖啡因可抑制钙超载的非洲爪蟾卵母细胞中的氯离子电流波动。

Caffeine suppresses chloride current fluctuations in calcium-overloaded Xenopus laevis oocytes.

作者信息

Poledna J, Packová V

机构信息

Institute of Molecular Physiology and Genetics, Slovak Academy of Sciences, Bratislava.

出版信息

Physiol Res. 1994;43(4):253-6.

PMID:7841172
Abstract

Calcium-induced chloride currents were studied in Xenopus oocytes using the two-electrode voltage clamp technique. Fluctuations of chloride currents measured under a voltage clamp were elicited by injection of calcium into the cytoplasm. Contrary to infrequent injections of small amounts of calcium which evoked smooth transient responses, these fluctuating chloride currents are due to overloading of intracellular calcium stores which then release calcium repeatedly. Chloride current fluctuations in calcium-overloaded oocytes can be reversibly suppressed by caffeine. This effect is concentration dependent and an amplitude decrease of fluctuations is already apparent at 2 mmol/l caffeine. The analysis of power spectra density of fluctuations have displayed the pronounced effect of caffeine. These results suggest that at least a part of the endoplasmic reticulum in Xenopus oocytes is a calcium-releasable calcium store which can be activated at the resting inositol trisphosphate concentration.

摘要

利用双电极电压钳技术在非洲爪蟾卵母细胞中研究了钙诱导的氯离子电流。通过向细胞质中注入钙引发在电压钳下测量的氯离子电流波动。与少量钙的不频繁注入引发平滑的瞬时反应相反,这些波动的氯离子电流是由于细胞内钙库过载,随后钙反复释放所致。钙超载卵母细胞中的氯离子电流波动可被咖啡因可逆性抑制。这种效应呈浓度依赖性,在2 mmol/l咖啡因时波动幅度就已明显降低。对波动的功率谱密度分析显示了咖啡因的显著作用。这些结果表明,非洲爪蟾卵母细胞中至少一部分内质网是可释放钙的钙库,在静息肌醇三磷酸浓度下即可被激活。

相似文献

1
Caffeine suppresses chloride current fluctuations in calcium-overloaded Xenopus laevis oocytes.咖啡因可抑制钙超载的非洲爪蟾卵母细胞中的氯离子电流波动。
Physiol Res. 1994;43(4):253-6.
2
Cytoplasmic calcium fluctuations in calcium overloaded Xenopus laevis oocytes.
Gen Physiol Biophys. 1995 Aug;14(4):339-47.
3
Calcium dependent variance of chloride current fluctuations in Xenopus laevis oocytes.非洲爪蟾卵母细胞中氯离子电流波动的钙依赖性变化
Gen Physiol Biophys. 1993 Oct;12(5):445-52.
4
Activation of different Cl currents in Xenopus oocytes by Ca liberated from stores and by capacitative Ca influx.从储存库释放的钙以及通过容量性钙内流在非洲爪蟾卵母细胞中激活不同的氯电流。
J Gen Physiol. 1996 Sep;108(3):157-75. doi: 10.1085/jgp.108.3.157.
5
Caffeine inhibits inositol trisphosphate-mediated liberation of intracellular calcium in Xenopus oocytes.咖啡因可抑制非洲爪蟾卵母细胞中肌醇三磷酸介导的细胞内钙释放。
J Physiol. 1991 Feb;433:229-40. doi: 10.1113/jphysiol.1991.sp018423.
6
Changes in intracellular calcium and in membrane currents evoked by injection of inositol trisphosphate into Xenopus oocytes.将肌醇三磷酸注入非洲爪蟾卵母细胞后引起的细胞内钙和膜电流变化。
Proc R Soc Lond B Biol Sci. 1986 Aug 22;228(1252):307-15. doi: 10.1098/rspb.1986.0057.
7
Analysis of calcium activated chloride current fluctuations in Xenopus laevis oocytes.
Gen Physiol Biophys. 1991 Jun;10(3):265-80.
8
Caffeine inhibits inositol-trisphosphate-induced membrane potential oscillations in Xenopus oocytes.咖啡因可抑制非洲爪蟾卵母细胞中肌醇三磷酸诱导的膜电位振荡。
Proc Biol Sci. 1991 Apr 22;244(1309):57-62. doi: 10.1098/rspb.1991.0051.
9
Modulation of Xenopus laevis Ca-activated Cl currents by protein kinase C and protein phosphatases: implications for studies of anesthetic mechanisms.蛋白激酶C和蛋白磷酸酶对非洲爪蟾钙激活氯电流的调节:对麻醉机制研究的启示
Anesth Analg. 2004 Aug;99(2):416-22, table of contents. doi: 10.1213/01.ANE.0000121351.38401.AB.
10
Interaction between capacitative Ca2+ influx and Ca2+-dependent Cl- currents in Xenopus oocytes.非洲爪蟾卵母细胞中容量性Ca2+内流与Ca2+依赖性Cl-电流之间的相互作用。
Pflugers Arch. 1996 Feb;431(4):954-63.

引用本文的文献

1
Wanderlust kinetics and variable Ca(2+)-sensitivity of dSlo [correction of Drosophila], a large conductance CA(2+)-activated K+ channel, expressed in oocytes.在卵母细胞中表达的大电导Ca(2+)激活的K+通道dSlo(果蝇校正)的漫游癖动力学和可变Ca(2+)敏感性
Biophys J. 1996 Jul;71(1):2640-51.
2
Wanderlust kinetics and variable Ca(2+)-sensitivity of Drosophila, a large conductance Ca(2+)-activated K+ channel, expressed in oocytes.果蝇中一种大电导钙激活钾通道在卵母细胞中表达时的漫游癖动力学和可变钙敏感性。
Biophys J. 1996 Jun;70(6):2640-51. doi: 10.1016/S0006-3495(96)79833-8.