Ricchelli F, Nikolov P, Gobbo S, Jori G, Moreno G, Salet C
C.N.R. Centre of Metalloproteins, University of Padova, Italy.
Biochim Biophys Acta. 1994 Dec 30;1196(2):165-71. doi: 10.1016/0005-2736(94)00230-4.
Absorption and emission spectroscopic studies on Zn(II)-phthalocyanine (ZnPc) incorporated into unilamellar liposomes of dipalmitoylphosphatidylcholine, sometimes added with cholesterol or cardiolipin, and released to rat liver mitochondria via the three types of liposomal vesicles indicated that: (a) ZnPc predominantly dissolves in all lipid domains of biological membranes with the exception of cardiolipin-containing regions; a partial localization of ZnPc in protein binding sites is also postulated; (b) the spectroscopic properties of ZnPc, although mainly determined by the aggregation state of the dye, are somewhat influenced by the physico-chemical characteristics of the lipid environment; (c) ZnPc-binding lipid domains in mitochondria are mainly localized in the outer membrane; this conclusion is clearly deduced from the trends of Arrhenius plots of the ZnPc fluorescence quantum yield in whole mitochondria and isolated inner or outer membrane in the temperature range -10 degrees C-(+)45 degrees C; (d) the nature of the ZnPc-binding site in mitochondria is not dependent on the chemical composition of the liposome carrier, contrary to what observed for other hydrophobic dyes, such as porphyrins. This has been also confirmed by photosensitization experiments. Actually, illumination of ZnPc-loaded mitochondria by 600-700 nm light causes a decline of the respiratory control ratio, which is essentially dependent on the amount of incorporated photosensitizer, irrespective of the composition of the carrier.
对掺入二棕榈酰磷脂酰胆碱单层脂质体（有时添加胆固醇或心磷脂）中的锌（II）-酞菁（ZnPc）进行的吸收和发射光谱研究表明，通过三种类型的脂质体囊泡将其释放到大鼠肝线粒体中：（a）ZnPc主要溶解在生物膜的所有脂质结构域中，但含心磷脂的区域除外；还推测ZnPc部分定位于蛋白质结合位点；（b）ZnPc的光谱性质虽然主要由染料的聚集状态决定，但在一定程度上受脂质环境的物理化学特性影响；（c）线粒体中与ZnPc结合的脂质结构域主要位于外膜；这一结论是根据在-10℃至45℃温度范围内整个线粒体以及分离的内膜或外膜中ZnPc荧光量子产率的阿伦尼乌斯图趋势明确推导出来的；（d）线粒体中ZnPc结合位点的性质不依赖于脂质体载体的化学组成，这与其他疏水性染料如卟啉的情况相反。这也通过光致敏实验得到了证实。实际上，用600 - 700 nm光照射负载ZnPc的线粒体导致呼吸控制率下降，这主要取决于掺入的光敏剂的量，而与载体的组成无关。
