Huang M, Albach R A, Chang K P, Tripathi R L, Kemp R G
Department of Microbiology/Immunology, Chicago Medical School, IL 60064.
Biochim Biophys Acta. 1995 Jan 25;1260(2):215-7. doi: 10.1016/0167-4781(94)00216-p.
Pyrophosphate-dependent phosphofructokinase (PPi-PFK) gene from Entamoeba histolytica was cloned from its genomic library and sequenced. The open reading frame has 1149 bp and codes for a protein of 41.5 kDa. The deduced amino acid sequence of E. histolytica PPi-PFK has 25 to 28% identity to the PPi-PFKs from Propionibacterium freudenreichii, Naegleria fowleri and potato. The amino acid residues known to contribute to the active site of PPi-PFK from P. freudenreichii are conserved.
从溶组织内阿米巴的基因组文库中克隆并测序了焦磷酸依赖性磷酸果糖激酶（PPi-PFK）基因。其开放阅读框有1149个碱基对，编码一个41.5 kDa的蛋白质。溶组织内阿米巴PPi-PFK推导的氨基酸序列与费氏丙酸杆菌、福氏耐格里阿米巴和马铃薯的PPi-PFK有25%至28%的同一性。已知对费氏丙酸杆菌PPi-PFK活性位点有贡献的氨基酸残基是保守的。
