Magazin M, Guillemot J C, Vita N, Ferrara P
Department of Protein Biochemistry, Sanofi Recherche, Labège, France.
Eur Cytokine Netw. 1994 Jul-Aug;5(4):397-400.
Recombinant human IL-13 is chemotactic for purified human peripheral blood monocytes Cell migration is stimulated with a typical bell-shaped concentration curve and is maximal at 10 ng/ml. Migration is the result of chemotaxis, and not chemokinesis, as shown by checkerboard experiments. The chemotactic activity of IL-13 on monocytes is not inhibited by preincubation of the cells with pertussis toxin but is diminished by preincubation with protein kinase inhibitors. The related cytokine, IL-4, also stimulates migration of monocytes in the Boyden chamber assays at concentrations similar to those effective for IL-13. Human IL-13 is capable of attracting rabbit peripheral blood monocytes at those concentrations active on human monocytes. On the other hand, no neutrophil migration was induced by IL-13, even at 1 microM concentrations.
重组人白细胞介素-13对纯化的人外周血单核细胞具有趋化作用。细胞迁移受到刺激，呈现典型的钟形浓度曲线，在10 ng/ml时达到最大值。棋盘实验表明，迁移是趋化作用的结果，而非细胞运动。用百日咳毒素预孵育细胞不会抑制白细胞介素-13对单核细胞的趋化活性，但用蛋白激酶抑制剂预孵育会使其活性降低。相关细胞因子白细胞介素-4在博伊登小室试验中，以与对白细胞介素-13有效的浓度相似的浓度刺激单核细胞迁移。人白细胞介素-13在对人单核细胞有活性的浓度下能够吸引兔外周血单核细胞。另一方面，即使在1 microM浓度下，白细胞介素-13也不会诱导中性粒细胞迁移。
