Selinger K
Glaxo Inc. Research Institute, Research Triangle Park, NC 27709.
Biomed Chromatogr. 1994 Sep-Oct;8(5):219-23. doi: 10.1002/bmc.1130080505.
A high-performance liquid chromatographic method for the determination of amiloride in airway surface liquid is described. It involves extraction of the drug with methanol from filter paper on which the sample is absorbed and chromatography on a Zorbax Rx column; the mobile phase is 25% acetonitrile in 0.05 M phosphate buffer; detection by fluorescence at 360/420 nm. Triamterene is used as an internal standard. The range of the assay is 2.0-2033.4 ng/sample, with adequate precision and accuracy. A power curve y = axb best describes the relationship between the peak-height ratio and concentration. Recovery of amiloride is non-linear, probably due to an adsorption process. Accuracy of the assay at lower amounts may be affected by the choice of filter paper a well as by presence of endogenous plasma components. The assay was used to measure amiloride amount in airway surface liquid after administration of 2.5 and 4.5 mg of nebulized amiloride.
本文描述了一种用于测定气道表面液体中阿米洛利的高效液相色谱法。该方法包括用甲醇从吸附有样品的滤纸上提取药物，并在Zorbax Rx柱上进行色谱分析；流动相为0.05 M磷酸盐缓冲液中25%的乙腈；在360/420 nm处进行荧光检测。氨苯蝶啶用作内标。该测定方法的范围为2.0 - 2033.4 ng/样品，具有足够的精密度和准确度。幂曲线y = axb最能描述峰高比与浓度之间的关系。阿米洛利的回收率是非线性的，可能是由于吸附过程。较低含量时测定的准确度可能会受到滤纸选择以及内源性血浆成分存在的影响。该测定方法用于测量雾化吸入2.5 mg和4.5 mg阿米洛利后气道表面液体中阿米洛利的含量。
