Tsai H J, Shih S R, Kuo C M, Li L K
Institute of Fisheries Sciences, National Taiwan University, Taipei.
Comp Biochem Physiol B Biochem Mol Biol. 1994 Sep;109(1):81-8. doi: 10.1016/0305-0491(94)90144-9.
A recombinant phage clone containing a 1584 nucleotides rhodopsin cDNA was screened from a carp retinal cDNA library. The inserted DNA consisting of a single open reading frame of 1062 nucleotides at positions 72 to 1133 encodes a 354 amino acid polypeptide. The deduced amino acid sequence of carp rhodopsin showed 95.7, 85.5 and 74.4% identity with that of goldfish, sand goby and lamprey, respectively. The sites of palmitoylation, glycosylation, disulfide bond formation and Schiff base formation in the putative rhodopsin are all conserved.
从鲤鱼视网膜cDNA文库中筛选出一个含有1584个核苷酸的视紫红质cDNA的重组噬菌体克隆。插入的DNA在72至1133位由一个1062个核苷酸的单一开放阅读框组成，编码一个354个氨基酸的多肽。推导的鲤鱼视紫红质氨基酸序列与金鱼、沙虎鱼和七鳃鳗的视紫红质氨基酸序列分别具有95.7%、85.5%和74.4%的同一性。假定的视紫红质中棕榈酰化、糖基化、二硫键形成和席夫碱形成的位点都是保守的。
