[丁型肝炎病毒中国株：分子克隆与测序及其基因组差异的意义]

[Chinese strain of hepatitis D virus: molecular cloning and sequencing, and the significance of its genome divergence].

作者信息

Zhou S L, Zhao L S, Lin Y

机构信息

Laboratory of Viral Hepatitis, West China Medical University, Chengdu.

出版信息

Zhonghua Yi Xue Za Zhi. 1994 Oct;74(10):605-7, 646.

PMID:7842336

Abstract

The hepatitis D virus (HDV) obtained from a chronic asymptomatic HDV/HBsAg carrier (SZ 93) in Sichuan province was cloned with 1,684 nucleotides in full-length genome by reverse transcription-PCR. Comparison of SZ 93 with those obtained from different geographic area: i.e., Italia, United States, British, Nauru, Taiwan, Netherland, showed 81.8%-95.4% homology in nucleotide sequence of whole genome, 88.9%-96.1% homology in nucleotide sequence of HDAg-coding region, and 86.4%-93.0% homology in amino acid sequence of HDAg protein. Also, another isolate (SZ 92) obtained from a chronic severe hepatitis patient in Sichuan was cloned with HDAg-coding region. There were some mutations in SZ 92 compared with SZ 93 as well as the other clones mentioned above. The meaning for the genome divergence of HDV SZ 93 and SZ 92 was discussed.

摘要

从四川省一名慢性无症状丁型肝炎病毒/乙肝表面抗原（HDV/HBsAg）携带者（SZ 93）体内获取的丁型肝炎病毒（HDV），通过逆转录聚合酶链反应（RT-PCR）克隆出全长基因组为1684个核苷酸的片段。将SZ 93与来自不同地理区域（即意大利、美国、英国、瑙鲁、台湾、荷兰）的毒株进行比较，结果显示全基因组核苷酸序列的同源性为81.8% - 95.4%，丁型肝炎抗原（HDAg）编码区核苷酸序列的同源性为88.9% - 96.1%，HDAg蛋白氨基酸序列的同源性为86.4% - 93.0%。此外，从四川省一名慢性重型肝炎患者体内获取的另一株分离株（SZ 92），克隆出了HDAg编码区。与SZ 93以及上述其他克隆株相比，SZ 92存在一些突变。文中还讨论了HDV SZ 93和SZ 92基因组差异的意义。