Liao H J, Chen X M, Cui S W
Department of Nephrology, General Hospital of PLA, Beijing.
Zhonghua Yi Xue Za Zhi. 1994 Oct;74(10):629-31, 648.
A multiparametric analysis was made to demonstrate that brief period of ischemia can initiate extensive loss in a rat kidney through the process of apoptosis during early reperfusion. Microscopic examination of mouse renal tissues subject to a 5, 30, or 45 minute period of complete ischemia showed the presence of apoptotic bodies both within and occasionally between renal tubular, appearing as early 6 hours after reperfusion and increasing in numbers at 12 hours. Furthermore, DNA extracted from such reperfusion renal tissue demonstrated the appearance of a distinct "ladder pattern" of DNA fragments after electrophoresis in agarose gels. It was suggested that renal reperfusion injury after ischemia can initiate a form of cell death-apoptosis that is drastically different from cellular necrosis induced by prolonged severe ischemia.
进行了多参数分析，以证明短暂的缺血期可在早期再灌注期间通过凋亡过程引发大鼠肾脏的广泛损失。对经历5、30或45分钟完全缺血的小鼠肾组织进行显微镜检查，结果显示肾小管内及偶尔肾小管之间存在凋亡小体，最早在再灌注后6小时出现，并在12小时时数量增加。此外，从这种再灌注肾组织中提取的DNA在琼脂糖凝胶中电泳后显示出独特的DNA片段“梯形图谱”。有人提出，缺血后的肾再灌注损伤可引发一种细胞死亡形式——凋亡，这与长期严重缺血诱导的细胞坏死截然不同。
