Czirják L, Molnár I, Csipö I, Szabolcs M, Mihály A, Szegedi G
3rd Department of Internal Medicine, University Medical School of Debrecen, Hungary.
Clin Exp Rheumatol. 1994 Sep-Oct;12(5):527-9.
An enzyme-linked immunosorbent assay and western immunoblotting analysis were used to detect anti-platelet antibodies in the sera of 50 patients with systemic sclerosis. Eleven (22%) positive sera were found by ELISA. Serial investigations showed that the presence of these antibodies was often transient. Immunoblotting analysis demonstrated that the antibodies were directed against a 114 kDa antigen. Using monoclonal antibody, anti-platelet antibodies directed against gpIIIa were found in 4 cases. Similar to immune thrombocytopenia and lupus erythematosus, a significant portion of the anti-platelet antibodies were also directed against the glycoprotein complex IIb/IIIa in systemic sclerosis.
采用酶联免疫吸附试验和蛋白质免疫印迹分析检测50例系统性硬化症患者血清中的抗血小板抗体。酶联免疫吸附试验发现11例（22%）血清呈阳性。连续检测显示这些抗体的存在通常是短暂的。免疫印迹分析表明这些抗体针对的是一种114 kDa的抗原。使用单克隆抗体，在4例患者中发现了针对糖蛋白IIIa的抗血小板抗体。与免疫性血小板减少症和红斑狼疮相似，系统性硬化症中相当一部分抗血小板抗体也针对糖蛋白复合物IIb/IIIa。
