Watanabe S, Kondo M, Takatsu K, Sugamura K, Arai K
Department of Molecular and Developmental Biology, University of Tokyo, Japan.
Eur J Immunol. 1995 Jan;25(1):126-31. doi: 10.1002/eji.1830250122.
Interleukin-4 (IL-4) has various activities on B cells and on hematopoietic cells. We previously reported that TUGm2, a monoclonal antibody to the gamma subunit of the IL-2 receptor (IL-2R gamma), inhibited IL-4-dependent proliferation of CTLL2, a cytotoxic T cell line. We proposed that IL-2R gamma is required for the functional IL-4 receptor (IL-4R) in T cells. In the present work, we further examined whether or not IL-2R gamma is involved in IL-4R function in mouse myeloid cell lines and splenic B cells. TUGm2 suppressed the IL-4-induced proliferation of BA/F3 or IC2 cells, as well as of purified splenic B cells. TUGm2 partially suppressed proliferation of B cells induced by the combination of IL-4 and anti-immunoglobulin M (IgM) antibody. In contrast, TUGm2 had no effect on proliferation of B cells induced by anti-IgM antibody alone or lipopolysaccharide (LPS). TUGm2 also inhibited IgE production induced by IL-4 of LPS-stimulated B cells. The induction of major histocompatibility complex class II molecules or CD23 by IL-4 was virtually unaffected by TUGm2 antibody. These results indicate that IL-2R gamma is differentially involved in various IL-4-dependent reactions.
白细胞介素-4（IL-4）对B细胞和造血细胞具有多种作用。我们之前报道过，TUGm2，一种针对白细胞介素-2受体（IL-2Rγ）γ亚基的单克隆抗体，可抑制细胞毒性T细胞系CTLL2的IL-4依赖性增殖。我们提出T细胞中功能性白细胞介素-4受体（IL-4R）需要IL-2Rγ。在本研究中，我们进一步检测了IL-2Rγ是否参与小鼠髓系细胞系和脾B细胞中的IL-4R功能。TUGm2可抑制IL-4诱导的BA/F3或IC2细胞以及纯化脾B细胞的增殖。TUGm2部分抑制了IL-4和抗免疫球蛋白M（IgM）抗体联合诱导的B细胞增殖。相比之下，TUGm2对单独抗IgM抗体或脂多糖（LPS）诱导的B细胞增殖没有影响。TUGm2还抑制了LPS刺激的B细胞经IL-4诱导产生IgE。TUGm2抗体几乎不影响IL-4诱导的主要组织相容性复合体II类分子或CD23的表达。这些结果表明IL-2Rγ以不同方式参与各种IL-4依赖性反应。
