Kawakami T, Matsumoto M, Sato M, Harada H, Taniguchi T, Kitagawa M
Institute for Molecular and Cellular Biology, Osaka University, Japan.
FEBS Lett. 1995 Jan 30;358(3):225-9. doi: 10.1016/0014-5793(94)01426-2.
Two virus-inducible transcription factors, IRF-1 and IRF-2 have been identified as an activator and a repressor, respectively, of the type I interferon (IFN) genes. Recent studies with mice carrying null mutations for the IRF-1 or IRF-2 alleles have revealed the existence of IRF-1-dependent and -independent pathways mediating IFN-beta gene induction. Here we report that the expression of an IRF family member ISGF3 gamma is induced upon viral infection in IRF-1-/-, IRF-2-/- embryonic fibroblasts. Furthermore, ISGF3 gamma can bind to a virus-inducible promoter element in the IFN-beta gene. These results suggest that ISGF3 gamma or complex containing ISGF3 gamma is involved in the IRF-1-independent pathway mediating IFN-beta gene regulation.
两种病毒诱导的转录因子，即IRF-1和IRF-2，已分别被鉴定为I型干扰素（IFN）基因的激活剂和抑制剂。最近对携带IRF-1或IRF-2等位基因无效突变的小鼠进行的研究表明，存在介导IFN-β基因诱导的IRF-1依赖性和非依赖性途径。在此我们报告，在IRF-1-/-、IRF-2-/-胚胎成纤维细胞中，病毒感染后IRF家族成员ISGF3γ的表达被诱导。此外，ISGF3γ可与IFN-β基因中的病毒诱导启动子元件结合。这些结果表明，ISGF3γ或包含ISGF3γ的复合物参与了介导IFN-β基因调控的IRF-1非依赖性途径。
