Mirecka J, Brinck U, Korabiowska M, Schauer A
Department of Histology, Medical Faculty, Collegium Medicum, Jagiellonian University, Kraków, Poland.
Folia Histochem Cytobiol. 1994;32(3):177-80.
Distribution of S-100 protein in porcine ovaries was revealed by an application of polyclonal antibodies against S-100 protein and PAP universal kit to formalin-fixed, paraffin-embedded tissues. The presence of S-100 protein was demonstrated in follicular cells of all ovarian follicles (including their atretic forms), with a decrease in intensity of the reaction parallel to an increase in follicle size. No reaction was found in oocytes, regardless of the developmental stage of the follicle. Weak expression of S-100 protein was observed in theca interna cells of mature follicles and in the youngest generation of theca-lutein cells of the corpus luteum. A distinctly positive reaction was also observed in groups of cells resembling hilus cells as well as in endothelia of arteries and some capillaries. Results are compared with data concerning the localization of S-100 protein in human and bovine ovaries. Possible relationship between the presence of S-100 protein and the level of cellular differentiation is discussed.
通过将抗S - 100蛋白的多克隆抗体和PAP通用试剂盒应用于福尔马林固定、石蜡包埋的组织，揭示了猪卵巢中S - 100蛋白的分布情况。在所有卵巢卵泡（包括其闭锁形式）的卵泡细胞中均证实存在S - 100蛋白，反应强度随卵泡大小增加而降低。无论卵泡的发育阶段如何，卵母细胞中均未发现反应。在成熟卵泡的内膜细胞和黄体最年轻一代的黄素化内膜细胞中观察到S - 100蛋白的弱表达。在类似门细胞的细胞群以及动脉和一些毛细血管的内皮中也观察到明显的阳性反应。将结果与关于S - 100蛋白在人及牛卵巢中定位的数据进行了比较。讨论了S - 100蛋白的存在与细胞分化水平之间可能的关系。
