Pastey M K, Samal S K
College of Veterinary Medicine, University of Maryland, College Park 20742.
J Gen Virol. 1995 Jan;76 ( Pt 1):193-7. doi: 10.1099/0022-1317-76-1-193.
The nucleotide and deduced amino acid sequences of the non-structural (NS) protein genes of bovine respiratory syncytial virus (BRSV) strain A51908 were determined. The NS1 and NS2 genes were 524 and 489 nucleotides long with single open reading frames, encoding polypeptides of 136 and 124 amino acids, respectively. Comparison of the NS1 gene of BRSV with the corresponding sequences of ovine respiratory syncytial virus (ORSV) and human respiratory syncytial virus (HRSV) subgroups A and B revealed 82, 67 and 65% identity at the nucleotide level, and 89, 69 and 68% identity at the amino acid level, respectively. The nucleotide identity of the NS2 gene of BRSV to those of ORSV and HRSV subgroups A and B was 80, 69 and 67% and the predicted amino acid identity was 87, 84 and 83%, respectively. The intergenic sequences of the NS1-NS2 and NS2-N gene junctions of BRSV were determined and found to be 13 nucleotides shorter and 29 nucleotides longer, respectively, than the corresponding regions of HRSV.
测定了牛呼吸道合胞病毒（BRSV）A51908株非结构（NS）蛋白基因的核苷酸序列和推导的氨基酸序列。NS1和NS2基因分别长524和489个核苷酸，具有单一开放阅读框，分别编码136和124个氨基酸的多肽。将BRSV的NS1基因与绵羊呼吸道合胞病毒（ORSV）以及人呼吸道合胞病毒（HRSV）A、B亚组的相应序列进行比较，结果显示在核苷酸水平上的同源性分别为82%、67%和65%，在氨基酸水平上的同源性分别为89%、69%和68%。BRSV的NS2基因与ORSV以及HRSV A、B亚组的核苷酸同源性分别为80%、69%和67%，预测的氨基酸同源性分别为87%、84%和83%。测定了BRSV的NS1 - NS2和NS2 - N基因连接处的基因间序列，发现其分别比HRSV的相应区域短13个核苷酸和长29个核苷酸。
