通过针对重组痘苗病毒载体表达的腮腺炎病毒（MuV）表面糖蛋白产生的抗体保护仓鼠免受实验性MuV感染。

Protection of hamsters against experimental mumps virus (MuV) infection by antibodies raised against the MuV surface glycoproteins expressed from recombinant vaccinia virus vectors.

作者信息

Houard S, Varsanyi T M, Milican F, Norrby E, Bollen A

机构信息

Service de Génétique Appliquée, University of Brussels, Nivelles, Belgium.

出版信息

J Gen Virol. 1995 Feb;76 ( Pt 2):421-3. doi: 10.1099/0022-1317-76-2-421.

DOI:10.1099/0022-1317-76-2-421

PMID:7844562

Abstract

The fusion (F) and haemagglutinin-neuraminidase (HN) glycoproteins of mumps virus (MuV) have been produced in CV1 cells via vaccinia virus recombinants. Recombinant proteins accumulated in infected cells and were glycosylated. Upon reduction, the F protein product was completely converted into its subunits. Hamsters infected with vaccinia recombinants expressing either the F or HN proteins produced antibodies recognizing MuV antigens and neutralizing MuV infectivity in vitro. These antibodies provided protection against MuV-induced encephalitis in newborn hamsters.

摘要

腮腺炎病毒（MuV）的融合（F）糖蛋白和血凝素神经氨酸酶（HN）糖蛋白已通过痘苗病毒重组体在CV1细胞中产生。重组蛋白在受感染细胞中积累并进行了糖基化。经还原后，F蛋白产物完全转化为其亚基。用表达F蛋白或HN蛋白的痘苗重组体感染的仓鼠产生了识别MuV抗原并在体外中和MuV感染性的抗体。这些抗体为新生仓鼠提供了针对MuV诱导的脑炎的保护作用。