Tsurudome M, Yamada A, Hishiyama M, Ito Y
J Gen Virol. 1986 Oct;67 ( Pt 10):2259-65. doi: 10.1099/0022-1317-67-10-2259.
Six monoclonal antibodies (MAbs) against the haemagglutinin-neuraminidase (HN) protein and three against the fusion (F) protein of mumps virus were obtained. Anti-HN MAbs were classified into three antigen-specific groups in competitive binding assays. All of the anti-HN MAbs showed virus-neutralizing and haemolysis-inhibiting activities and inhibited fusion from without (FFWO). Three of them inhibited fusion from within (FFWI) and the cell-to-cell spread of infection. The most effective MAb in inhibiting FFWI possessed neuraminidase-inhibiting activity and little haemagglutination-inhibiting activity. All anti-F MAbs inhibited virus-induced haemolysis but none of them inhibited FFWO, FFWI or the cell-to-cell spread of infection.
获得了六种针对腮腺炎病毒血凝素神经氨酸酶（HN）蛋白的单克隆抗体（MAb）和三种针对融合（F）蛋白的单克隆抗体。在竞争结合试验中，抗HN单克隆抗体被分为三个抗原特异性组。所有抗HN单克隆抗体均表现出病毒中和及溶血抑制活性，并抑制从外融合（FFWO）。其中三种抑制从内融合（FFWI）及感染的细胞间传播。抑制FFWI最有效的单克隆抗体具有神经氨酸酶抑制活性且几乎没有血凝抑制活性。所有抗F单克隆抗体均抑制病毒诱导的溶血，但均不抑制FFWO、FFWI或感染的细胞间传播。
